Imperial/Infector Detector/Implementation

From 2007.igem.org

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|width="40%"| Not Required
|width="40%"| Not Required
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|width="40%"| [[Image:Purification_of_LuxR.PNG|center|300px]] LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results])
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|width="40%"| [[Image:Purification_of_LuxR.PNG|center|thumb|200px|Purification of LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.5 Results])]]
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br>[[Image:Icegems-Devcycle-downarrow.png|30px]]

Revision as of 01:39, 26 October 2007



Infector Detector: Implementation

DNA Construct Implementation
Construct Design
IC07 design ID.png
IC07 design ID-con2.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png
Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry.
Imperial ID Design ID1.png
Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry.
Imperial ID Design ID2.png
Stage 2: Cloning / Amplification

Icegems-Devcycle-downarrow.png
BBa_T9002 - Lane 18 (1945bp)
BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation

Icegems-Devcycle-downarrow.png
Not Required Not Required
Stage 4: Purification of Activator Proteins

Icegems-Devcycle-downarrow.png
Not Required
Purification of LuxR protein (Protocol and Results)
Stage 5: Quality Control

Icegems-Devcycle-downarrow.png
IC 2007 Plux in vivo.PNG

  • Testing in vivo: Passed (Protocol and Results)
  • Sequencing of Construct: Completed
IC 2007 LuxR testing 1.PNG

  • Testing in vitro: Failed (Protocol and Results)
  • Sequencing of Construct: Completed
Stage 6: Final Implementation Status Ready for Testing Aborted.
LuxR protein not functional.



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png
Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation

Icegems-Devcycle-downarrow.png
Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control

Icegems-Devcycle-downarrow.png
  • Testing in vitro: Failed ([[Protocol and Results)
Stage 4: Final Implementation Status Aborted.
Expression capacity of chassis was low.
Ready for Testing



<< Modelling | Implementation | Testing/Validation >>