From 2007.igem.org

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

Fabrication Protocols

Testing Protocols

Construct Protocols Cloning of DNA- MiniPrep Cloning of DNA- MidiPrep Cloning of DNA- MaxiPrep Protein Purification Purification of LuxR Testing of Purified LuxR Purification of GFPmut3b GFPmut3b Calibration Curve Fluorometer Testing Optimsation of Fluorometer Measuring and Preventing Evaporation?? Wrong. Testing How Fluorescence Changes With Volume Calibration Curve for DsRed express Degradation Curve (For GFP? Non-existent) DNA concentration experiments Chassis Protocols E. coli Extracts Preparation of S30 E. coli extract Preparation of S12 E. coli extract Preparation of reaction mixture Vesicles Formation Vesicles Formation 2

Cell by Date Implementation Initial Testing for DNA constructs of CBD In Vivo Initial Testing for DNA constructs of CBD In Vitro (Chassis) Testing/Validation Operating Temperature Range (Initial Tests) Operating Temperature Range (Refined Tests) Infector Detector Implementation Initial Testing for DNA constructs of ID In Vivo Initial Testing for DNA constructs of ID In Vitro (Chassis) Testing/Validation Preliminary AHL Testing AHL Sensitivity: Real Life Applications Final AHL Testing (Standardized)