From 2007.igem.org

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

Fabrication Protocols

Testing Protocols

Construct Protocols Cloning of DNA- MiniPrep Cloning of DNA- MidiPrep Cloning of DNA- MaxiPrep Protein Purification Purification of LuxR Testing of Purified LuxR Results Purification of GFPmut3b GFPmut3b Calibration Curve (Wrong, this is the recombinant GFP Fluorometer Testing Optimisation of Fluorometer Results Measuring and Preventing Evaporation?? Wrong. Testing How Fluorescence Changes With Volume Results Calibration Curve for DsRed express Degradation Curve (For GFP? Non-existent) Chassis Protocols E. coli Extracts Preparation of S30 E. coli extract Preparation of S12 E. coli extract Preparation of reaction mixture.. what is this actually?? Vesicles Formation Vesicles Formation

Cell by Date Implementation Initial Testing for DNA constructs of CBD In Vivo Results Initial Testing for DNA constructs of CBD In Vitro (Chassis) Results Testing/Validation Operating Temperature Range (Initial Tests) Operating Temperature Range (Refined Tests) Results + ones for initial operating range Results for pT7 Detemining System Capabilities - DNA concentration Results Packaging protocol?? Results are in OWW. Steps protocol?? Results are in OWW. Infector Detector Implementation Initial Testing for DNA constructs of ID In Vivo Results Initial Testing for DNA constructs of ID In Vitro (Chassis) Results Testing/Validation Preliminary AHL Testing Results Results AHL Sensitivity: Real Life Applications Results Final AHL Testing (Standardized)