Protein Purification

• Purification of LuxR : Protocol and Results
• Purification of GFPmut3b: Protocol and Results

Fluorometer Testing

• Optimisation of Fluorometer: Protocol and Results

Calibration Curve

• Calibration Curve for GFPmut3b Protocol and Results
• Degradation Curve for GFPmut3b Protocol and Results

Chassis Protocols

• Preparation S30 Cell Extract Protocol
• Preparation S12 Cell Extract Protocol
• Preparation of Reaction Mixture Protocol
• Vesicles Formation Vesicles Formation

Cell by Date
Implementation

• In Vivo Testing: Protocols and Results
• Initial In vitro Testing:Protocols
  • Results

Testing/Validation

• Operating Temperature Range (Initial Tests)
• Operating Temperature Range (Refined Tests)
  • Results + ones for initial operating range
  • Results for pT7
• Detemining System Capabilities - DNA concentration
  • Results
• Packaging protocol?? Results are in OWW.
• Steps protocol?? Results are in OWW.

Infector Detector
Implementation

• Initial Testing for DNA constructs of ID In Vivo
  • Results
• Initial Testing for DNA constructs of ID In Vitro (Chassis)
  • Results

Testing/Validation

• Final AHL Testing
• Testing of Purified LuxR
  • Results