From 2007.igem.org

yesterday -- tomorrow

Contents 1 PCR on Colony 2 Preparing competent FX85 cells for Recombination Rate Test 3 Recombination Rate Test 4 Culture of FR781 for FACS

PCR on Colony

• L52 (O18 & O7) ---> give nothing
• L53 (O18 & O7) ---> give nothing
• L55 (O18 & O9) ---> clone 14 & 16 OK

Preparing competent FX85 cells for Recombination Rate Test

• 1- Classic protocol for preparing TSS chemically competent bacteria
  
• 2- Classic transformation protocol (1µL of Miniprep), except that we don't use an expression period before starting to put the antibiotics (Kan and Amp)
  
• 3- We

Recombination Rate Test

Culture of FR781 for FACS