Paris/August 30
From 2007.igem.org
< Paris(Difference between revisions)
| (13 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
== Expression test of DapA promotor by differential DAP repression == | == Expression test of DapA promotor by differential DAP repression == | ||
| + | |||
| + | == Plasmids: MiniPrep Products == | ||
| + | {| | ||
| + | |- style="background: #ccccff;" | ||
| + | ! colspan="5" style="background: #ccccff;" | Plasmids: MiniPrep Products | ||
| + | |- style="background: #ccccff; text-align: center;" | ||
| + | |width=5%| Number | ||
| + | |width=25%| Name | ||
| + | |width=50%| Description | ||
| + | |width=15%| Products in use | ||
| + | |width=5%| Date | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP15 | ||
| + | |Att B | ||
| + | |Lambda Att B rev within pSB2K3 | ||
| + | | | ||
| + | | [[Paris/August 30| August 30]] | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |MP16 | ||
| + | |Att P | ||
| + | |Lambda Att P within pSB2K3 | ||
| + | | | ||
| + | | [[Paris/August 30| August 30]] | ||
| + | |} | ||
| + | |||
| + | == Strains (glycerol stock) == | ||
| + | {| | ||
| + | |- style="background: #ccccff;" | ||
| + | ! colspan="5" style="background: #ccccff;" | Strains glycerol stock | ||
| + | |- style="background: #ccccff; text-align: center;" | ||
| + | |width=5%| Number | ||
| + | |width=25%| Name | ||
| + | |width=55%| Description | ||
| + | |width=5%| Verified clone | ||
| + | |width=5%| Date | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |S46 | ||
| + | |MP15 | ||
| + | |Lambda Att B rev within pSB2K3 | ||
| + | | | ||
| + | |[[Paris/August 30| August 30]] | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |S47 | ||
| + | |MP16 | ||
| + | |Lambda Att P within pSB2K3 | ||
| + | | | ||
| + | |[[Paris/August 30| August 30]] | ||
| + | |} | ||
| + | |||
| + | == Digestions == | ||
| + | we digest: | ||
| + | |||
| + | * MP1.1 as Bw vector with Spe1 & Pst1 | ||
| + | * MP15 as Bw insert with Xba1 & Pst1 | ||
| + | * MP16 as Bw insert with Xba1 & Pst1 | ||
| + | |||
| + | Protocol:<br> | ||
| + | ADN: 20µL<br> | ||
| + | BSA: 0.5µL<br> | ||
| + | NE Buffer 2: 5µL<br> | ||
| + | Enzyme 1: 2µL<br> | ||
| + | Enzyme 2: 2µL<br> | ||
| + | H2O: 20.5µL<br> | ||
Latest revision as of 12:02, 31 August 2007
Contents |
Expression test of DapA promotor by differential DAP repression
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP15 | Att B | Lambda Att B rev within pSB2K3 | August 30 | |
| MP16 | Att P | Lambda Att P within pSB2K3 | August 30 | |
Strains (glycerol stock)
| Strains glycerol stock | ||||
|---|---|---|---|---|
| Number | Name | Description | Verified clone | Date |
| S46 | MP15 | Lambda Att B rev within pSB2K3 | August 30 | |
| S47 | MP16 | Lambda Att P within pSB2K3 | August 30 | |
Digestions
we digest:
- MP1.1 as Bw vector with Spe1 & Pst1
- MP15 as Bw insert with Xba1 & Pst1
- MP16 as Bw insert with Xba1 & Pst1
Protocol:
ADN: 20µL
BSA: 0.5µL
NE Buffer 2: 5µL
Enzyme 1: 2µL
Enzyme 2: 2µL
H2O: 20.5µL
