Imperial/Cell-Free/Contribution
From 2007.igem.org
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__NOTOC__ | __NOTOC__ | ||
| - | == ''In vitro'' Expression == | + | == Our Contribution == |
| + | === ''In vitro'' Expression === | ||
*Attempted to make S30 ''E. coli'' cell extract and feeding solution | *Attempted to make S30 ''E. coli'' cell extract and feeding solution | ||
*Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP | *Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP | ||
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| - | == Vesicles Formation == | + | === Vesicles Formation === |
*Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer | *Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer | ||
*Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract | *Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract | ||
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| - | == Documentation on Registry of Parts == | + | === Documentation on Registry of Parts === |
Documented our findings with the following Registry Additions: | Documented our findings with the following Registry Additions: | ||
* 1 | * 1 | ||
* 2 | * 2 | ||
* 3 | * 3 | ||
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| + | |||
| + | <center> [https://2007.igem.org/Imperial/Cell-Free/Comparison << Cell-Free vs. Cell] | Our Contribution | [https://2007.igem.org/Imperial/Cell-Free/Characterisation Characterisation >>] | ||
| + | </center> | ||
Revision as of 22:00, 20 October 2007
Our Contribution
In vitro Expression
- Attempted to make S30 E. coli cell extract and feeding solution
- Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
- Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
- Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution
Vesicles Formation
- Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
- Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
- Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
- Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
Documentation on Registry of Parts
Documented our findings with the following Registry Additions:
- 1
- 2
- 3
