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	== Optimal DNA Concentration ==		== Optimal DNA Concentration ==
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		+	<center> [https://2007.igem.org/Imperial/Cell-Free/Contribution <<  Our Contribution ] | Characterisation | [https://2007.igem.org/Imperial/Cell-Free/Applications Applications >>]
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Revision as of 22:02, 20 October 2007

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Introduction

The characterisation of parts and devices in the [http://partsregistry.org/Main_Page Registry of Standard Biological Parts] have have been considered in terms of rate of gene expression in the units Polymerase per Second (PoPS) or Ribosome per Second (RiPS). However the characterisation of the cell-free chassis housing the expression machinery has not been considered up till now. Using [http://www.promega.com Promega's] commercial S30 E. coli cell extract, our team has investigated some parameters that will help us to better understand the characteristics and limitations associated with cell-free systems.

Operating Temperature Ranges

Optimal DNA Concentration

<< Our Contribution | Characterisation | Applications >>