Imperial/Cell-Free/Characterisation
From 2007.igem.org
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| - | == | + | == Cell-Free: Characterisation == |
The characterisation of parts and devices in the [http://partsregistry.org/Main_Page Registry of Standard Biological Parts] have have been considered in terms of rate of gene expression in the units Polymerase per Second (PoPS) or Ribosome per Second (RiPS). However the characterisation of the cell-free chassis housing the expression machinery has not been considered up till now. Using [http://www.promega.com Promega's] commercial S30 ''E. coli'' cell extract, our team has investigated some parameters that will help us to better understand the characteristics and limitations associated with cell-free systems. | The characterisation of parts and devices in the [http://partsregistry.org/Main_Page Registry of Standard Biological Parts] have have been considered in terms of rate of gene expression in the units Polymerase per Second (PoPS) or Ribosome per Second (RiPS). However the characterisation of the cell-free chassis housing the expression machinery has not been considered up till now. Using [http://www.promega.com Promega's] commercial S30 ''E. coli'' cell extract, our team has investigated some parameters that will help us to better understand the characteristics and limitations associated with cell-free systems. | ||
| - | == Operating Temperature Ranges == | + | === Operating Temperature Ranges === |
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| - | == Optimal DNA Concentration == | + | === Optimal DNA Concentration === |
Revision as of 22:21, 20 October 2007
Cell-Free: Characterisation
The characterisation of parts and devices in the [http://partsregistry.org/Main_Page Registry of Standard Biological Parts] have have been considered in terms of rate of gene expression in the units Polymerase per Second (PoPS) or Ribosome per Second (RiPS). However the characterisation of the cell-free chassis housing the expression machinery has not been considered up till now. Using [http://www.promega.com Promega's] commercial S30 E. coli cell extract, our team has investigated some parameters that will help us to better understand the characteristics and limitations associated with cell-free systems.
Operating Temperature Ranges
Optimal DNA Concentration
