From 2007.igem.org

Revision as of 01:33, 22 October 2007

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Cell-Free: Characterisation

The characterisation of parts and devices in the [http://partsregistry.org/Main_Page Registry of Standard Biological Parts] have have been considered in terms of rate of gene expression in the units Polymerase per Second (PoPS) or Ribosome per Second (RiPS). However the characterisation of the cell-free chassis housing the expression machinery has not been considered up till now. Our team has defined some parameters as well as partially characterized the various systems that will help us better understand the characteristics and limitations associated with CFS in general.

For a definition of chassis specifications, click [http://partsregistry.org/Chassis/Cell-Free_Systems here].

Follow the links below for more information on their characterisation:

• [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade E. coli S30]
• [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial E. coli S30]
• [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial E. coli T7 S30]
• [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]