Imperial/Infector Detector/Specification

From 2007.igem.org

(Difference between revisions)
(→Specifications in detail)
(→Infector Detector: Specifications)
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In order for the system to be used by a nurse, without needing any special equipment, the output signal must be visible.
In order for the system to be used by a nurse, without needing any special equipment, the output signal must be visible.
* '''Response Time: < 3 hours'''
* '''Response Time: < 3 hours'''
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Given that biofilms grow and spread in a period of hours and days, the response time of the system must be as short as possible. The threshold of three hours is achievable in the time constraints of protein expression systems, yet it is short enough in comparison to the growth properties of biofilms.
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Given that biofilms grow and spread in a period of hours and days, the response time of the system must be as short as possible. The threshold of three hours is achievable in the time constraints of protein expression systems, yet it is short enough in comparison to the growth properties of biofilms. ([[#References|2-3]])
* '''Operating conditions: Temperatures from 20&deg; to 30&deg;C'''
* '''Operating conditions: Temperatures from 20&deg; to 30&deg;C'''
* '''Health & safety: Non-living, non-infectious'''
* '''Health & safety: Non-living, non-infectious'''
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# Charlton, TS, et al. A novel and sensitive method for the quantification of N-3-oxoacyl homoserine lactones using gas chromatography–mass spectrometry: application to a model bacterial biofilm. Environmental Microbiology 2 (5), 530–541. October 2000.
# Charlton, TS, et al. A novel and sensitive method for the quantification of N-3-oxoacyl homoserine lactones using gas chromatography–mass spectrometry: application to a model bacterial biofilm. Environmental Microbiology 2 (5), 530–541. October 2000.
 +
# Stickler, DJ, et al. A Sensor To Detect the Early Stages in the Development of Crystalline Proteus mirabilis Biofilm on Indwelling Bladder Catheters. Journal of Clinical Microbiology, April 2006, p. 1540-1542, Vol. 44, No. 4.
 +
# [http://inls.ucsd.edu/~volfson/biofilm/ Growth and ordering of biofilms in controlled environments] available online on 22.10.2007

Revision as of 22:18, 22 October 2007


Infector Detector: Specifications

The system must be able to detect the presence of biofilms on urinary catheters by detection of AHL, at a minimum concentration of 5nM, and report with a visual signal within 3 hours. It must work within a temperature range of 20°-30°C, be portable and easy to use, have a shelf life of at least seven days, and must not be harmful or infectious.


Property
Value
Inputs
System must be sensitive to AHL concentration between 5-50nM
Outputs
System must give a visual signal if bacteria is present
Response Time
System needs to have a response time under 3 hour
Operating Conditions
System must operate within temperature 20-30°C
Health & Safety
System Must not be living replicating bacteria, and in any way harmful or infectious.
Lifespan
System must have a shelf life of 7 days
Packaging
System must be portable and convenient to use


Specifications in detail

Desired response of Infector Detector. Note that the response is not necessarily linear.
  • Input: AHL 5-50nM

It is known that, in Pseudomonas aeruginosa biofilms, the concentration of AHL is at least 5nM. (1) Therefore, if the system can successfully report the presence of this concentration, it should be able to detect such biofilms.

  • Output: Visible fluorescent protein

In order for the system to be used by a nurse, without needing any special equipment, the output signal must be visible.

  • Response Time: < 3 hours

Given that biofilms grow and spread in a period of hours and days, the response time of the system must be as short as possible. The threshold of three hours is achievable in the time constraints of protein expression systems, yet it is short enough in comparison to the growth properties of biofilms. (2-3)

  • Operating conditions: Temperatures from 20° to 30°C
  • Health & safety: Non-living, non-infectious
  • Packaging and shelf-life of 7 days


References

  1. Charlton, TS, et al. A novel and sensitive method for the quantification of N-3-oxoacyl homoserine lactones using gas chromatography–mass spectrometry: application to a model bacterial biofilm. Environmental Microbiology 2 (5), 530–541. October 2000.
  2. Stickler, DJ, et al. A Sensor To Detect the Early Stages in the Development of Crystalline Proteus mirabilis Biofilm on Indwelling Bladder Catheters. Journal of Clinical Microbiology, April 2006, p. 1540-1542, Vol. 44, No. 4.
  3. [http://inls.ucsd.edu/~volfson/biofilm/ Growth and ordering of biofilms in controlled environments] available online on 22.10.2007
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