Imperial/Cell by Date/Implementation

From 2007.igem.org

(Difference between revisions)
(→Cell by Date: Implementation)
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|- align="center"
|- align="center"
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
-
|width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]]
+
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]]
-
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]]
+
|width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]]
|- align="center"
|- align="center"
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
|width="40%"| Not Required
|width="40%"| Not Required
-
|width="40%"| Not Required
+
|width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
 +
<br>
 +
{| width="80%"
 +
|-
 +
|[[Image:Imperial_CBD_Design_CBD4.png|30px]]
 +
|<font size="+3">+</font>
 +
|[[Image:Imperial_CBD_Design_CBD3.png|110px]]
 +
|<font size="+3">=</font>
 +
|[[Image:Imperial CBD Design CBD2.png|130px]]
 +
|}
|- align="center"
|- align="center"
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
|width="40%"| Not Required
|width="40%"| Not Required
-
|width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results])
+
|width="40%"| Not Required
|-  
|-  
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
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* '''Sequencing of Construct''': Pending
* '''Sequencing of Construct''': Pending
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results])  
+
* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results])  
* '''Sequencing of Construct''': Pending
* '''Sequencing of Construct''': Pending
|- align="center"
|- align="center"
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
|width="40%" style="background-color:#b8efb0"| Ready for Testing
|width="40%" style="background-color:#b8efb0"| Ready for Testing
-
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> LuxR protein not functional.
+
|width="40%" style="background-color:#b8efb0"| Ready for Testing
|}
|}
<br clear="all">
<br clear="all">

Revision as of 13:25, 24 October 2007


Cell by Date: Implementation

DNA Construct Implementation
Construct Design Imperial CBD Design CBD1.png Imperial CBD Design CBD2.png
Stage 1: Availability Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
Stage 2: Cloning / Amplification
BBa_I13522 - Lane R2 (937bp + 2079bp vector)
BBa_E7104 - Lane 11 (Missing 826bp)
Stage 3: Ligation Not Required Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.png
Stage 4: Purification of Activator Proteins Not Required Not Required
Stage 5: Quality Control
  • Testing in vivo: Done (Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results])
  • Sequencing of Construct: Pending
  • Testing in vivo: Done (Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results])
  • Sequencing of Construct: Pending
Stage 6: Final Implementation Status Ready for Testing Ready for Testing









DNA Construct Status

Imperial CBD Design CBD1.png
Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
Imperial CBD Design CBD2.png
Notes: The part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Constitutive tetR Promoter

Imperial CBD Design CBD1.png

Ligation Strategy:

N/A

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at the testing page.
 Sequencing Pending Sequence results not obtained yet

Constitutive T7 Promoter

Imperial CBD Design CBD2.png

Ligation Strategy:

Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.png

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at -----
 Sequencing Pending Sequence results not obtained yet


Chassis Status

cell extract preparation protocols.

Navigation

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