Revision as of 00:15, 25 October 2007

Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments

Testing Experiments

Protein Purification

Fluorometer Testing

Calibration Curve

Chassis Protocols

pT7 Characterisation

Cell by Date
Implementation

Testing/Validation

Testing DNA Concentration in vitro: Protocols and Results
Operating Temperature Range: Protocol 1 and Protocol 2 and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.2 Results 1] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
Temperature Steps: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]

Infecter Detector
Implementation

In vivo Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]
Initial In Vitro Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]

Testing/Validation

Testing of DNA concentration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res2.1 Results]
Testing of Purified LuxR: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]
Characterization in vitro - AHL titration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 Results]

@@ Line 37: / Line 37: @@
 * ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.2|Results]]
 <font color=green>'''Testing/Validation<br>'''</font>
-*''Testing DNA Concentration in vitro'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD4 Results]
+*''Testing DNA Concentration in vitro'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
 * ''Operating Temperature Range'': [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.2 Results 1] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
 *''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]