Imperial/Wet Lab/Protocols

From 2007.igem.org

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<font color=green>'''Testing/Validation<br>'''</font>
<font color=green>'''Testing/Validation<br>'''</font>
*''Testing DNA Concentration in vitro'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
*''Testing DNA Concentration in vitro'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
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* ''Operating Temperature Range'': [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.2 Results 1] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
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* ''Operating Temperature Range'': [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results 1]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]
*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]
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Revision as of 13:06, 25 October 2007


Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols


pT7 Characterisation


Cell by Date
Implementation

Testing/Validation

  • Testing DNA Concentration in vitro: Protocols and Results
  • Operating Temperature Range: Protocol 1 and Protocol 2 and Results 1 and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
  • Temperature Steps: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]


Infecter Detector
Implementation

  • In vivo Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]
  • Initial In Vitro Testing: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]

Testing/Validation

  • Testing of DNA concentration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res2.1 Results]
  • Testing of Purified LuxR: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]
  • Characterization in vitro - AHL titration : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 Results]