Imperial/Infector Detector/Testing
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== Aims of Testing== | == Aims of Testing== | ||
- | To test and characterise the key characteristics of our system such as the sensitivity of the system to AHL. To do this, we induce the system with known concentrations of AHL input and measure the fluorescence output. Then using a calibration curve the fluorescence was converted into the number of GFPmut3b molecules synthesised, click on the following link for an explaination about how to [ | + | To test and characterise the key characteristics of our system such as the sensitivity of the system to AHL. To do this, we induce the system with known concentrations of AHL input and measure the fluorescence output. Then using a calibration curve the fluorescence was converted into the number of GFPmut3b molecules synthesised, click on the following link for an explaination about how to [[Imperial/Wet Lab/Results/Res1.3/Converting_Units| use calibration curve]] use the calibration curve]. The full results and protocols can be found on the links [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 results] and [[Imperial/Wet_Lab/Protocols/ID3.1|protocol]] pages. |
=Results= | =Results= |
Revision as of 01:10, 26 October 2007
Infector Detector: Testing
Aims of Testing
To test and characterise the key characteristics of our system such as the sensitivity of the system to AHL. To do this, we induce the system with known concentrations of AHL input and measure the fluorescence output. Then using a calibration curve the fluorescence was converted into the number of GFPmut3b molecules synthesised, click on the following link for an explaination about how to use calibration curve use the calibration curve]. The full results and protocols can be found on the links [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 results] and protocol pages.
Results
The results show us the following:
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