Imperial/Cell-Free/Contribution
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| + | <link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /> | ||
| - | == | + | <div id="tabs"> |
| + | <ul> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Whatis" title=""><span>What is Cell-Free?</span></a></li> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Comparison" title=""><span>Advantages of CFS</span></a></li> | ||
| + | <li><a class="current" href="https://2007.igem.org/Imperial/Cell-Free/Contribution" title=""><span>Our Contributions</span></a></li> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Characterisation" title=""><span>Characterisation</span></a></li> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Applications" title=""><span>CFS Applications</span></a></li> | ||
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| + | = Cell-Free: Our Contribution = | ||
| + | |||
| + | == ''In vitro'' Expression == | ||
| + | *Attempted to make S30 ''E. coli'' cell extract and feeding solution | ||
| + | *Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP | ||
| + | *Successfully used homemade S30 ''E. coli'' cell extract with commercial feeding solution for expression of GFP | ||
| + | *Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 ''E. coli'' cell extract and feeding solution | ||
== Vesicles Formation == | == Vesicles Formation == | ||
| + | *Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer | ||
| + | *Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract | ||
| + | *Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs | ||
| + | *Attempting to find suitable pore proteins to prolong vesicle and expression lifespan | ||
| + | == Documentation on Registry of Parts == | ||
| + | Documented our findings with the following Registry Additions: | ||
| + | [http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems] | ||
| + | * [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade ''E. coli'' S30] | ||
| + | * [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial ''E. coli'' S30] | ||
| + | * [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial ''E. coli'' T7 S30] | ||
| + | * [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation] | ||
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| + | <center> [https://2007.igem.org/Imperial/Cell-Free/Comparison << Advantages of CFS] | Our Contribution | [https://2007.igem.org/Imperial/Cell-Free/Characterisation Characterisation >>] | ||
| + | </center> | ||
Latest revision as of 19:55, 26 October 2007
Cell-Free: Our Contribution
In vitro Expression
- Attempted to make S30 E. coli cell extract and feeding solution
- Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
- Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
- Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution
Vesicles Formation
- Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
- Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
- Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
- Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
Documentation on Registry of Parts
Documented our findings with the following Registry Additions:
[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade E. coli S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial E. coli S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial E. coli T7 S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]
