Imperial/Wet Lab/Results/Res1.3

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= Calibration Curve: GFPmut3b ''in vitro'' vs Fluorescence=
= Calibration Curve: GFPmut3b ''in vitro'' vs Fluorescence=
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__NOTOC__
 
==Aims==
==Aims==
To construct a calibration curve of GFPmut3b in the in vitro chassis to enable the conversion of Fluorescence to more useful units.
To construct a calibration curve of GFPmut3b in the in vitro chassis to enable the conversion of Fluorescence to more useful units.
==Materials and Methods==
==Materials and Methods==
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Link to the [https://2007.igem.org/Imperial/Wet_Lab/Protocols/Prot1.3 Protocol]
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Link to the [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]]
==Results==
==Results==
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==Conclusion==
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'''Raw Data'''
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Now that we have a calibration curve in terms of many units of fluorescene, it is possible for us to convert the results got in terms of fluorescence to molecules produced. This would make the results more generic and thus the transfer function of the constructs can be obtained which is independant of the reporter attached at the end of the construct.
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*[[Media:Copy of FINAL CALIBRATION CURVE (edit).xls| Raw Data excel file ]]
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<br>The conversion from one unit to another can be done using this [[IGEM:IMPERIAL/2007/new pages/Data anaylsis|conversion method]]
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==Discussion==
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The results obtained fit a linear plot very well.
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==Conclusion==
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Now that we have a calibration curve in terms of units of fluorescence, it is possible for us to convert the results obtained in terms of fluorescence to molecules produced. This would make the results more generic and thus the transfer function of the constructs can be obtained which is independant of the reporter attached at the end of the construct.
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'''Raw Data'''
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<br>The conversion from one unit to another can be done using this [[Imperial/Wet Lab/Results/Res1.3/Converting Units|conversion method]].
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*[[Media:Copy of FINAL CALIBRATION CURVE (edit).xls| Raw Data excel file ]]
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Latest revision as of 02:12, 27 October 2007



Calibration Curve: GFPmut3b in vitro vs Fluorescence

Aims

To construct a calibration curve of GFPmut3b in the in vitro chassis to enable the conversion of Fluorescence to more useful units.

Materials and Methods

Link to the Protocol

Results


Figure 1.1 - Concentration vs Fluorescence

Figure 1.2 - Moles vs Fluorescence

Figure 1.3 - Molecules vs Fluorescence

Figure 1.4 - Molecules per plasmid vs Fluorescence

Raw Data

Discussion

The results obtained fit a linear plot very well.

Conclusion

Now that we have a calibration curve in terms of units of fluorescence, it is possible for us to convert the results obtained in terms of fluorescence to molecules produced. This would make the results more generic and thus the transfer function of the constructs can be obtained which is independant of the reporter attached at the end of the construct.


The conversion from one unit to another can be done using this conversion method.

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