Imperial/Cell by Date/Implementation
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|wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design''' | |wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design''' | ||
| - | |width="40%"| '''Concept 1'''<br />[[Image: | + | |width="40%"| '''Concept 1'''<br />[[Image:IC07_design_CBDcon1.png|center|370px]] |
| - | |width="40%"| '''Concept 2'''<br />[[Image: | + | |width="40%"| '''Concept 2'''<br />[[Image:IC07_design_CBDcon2.png|center|370px]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
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{| width="80%" | {| width="80%" | ||
|- | |- | ||
| - | |[[Image:Imperial_CBD_Design_CBD4.png| | + | |[[Image:Imperial_CBD_Design_CBD4.png|37px]] |
|<font size="+3">+</font> | |<font size="+3">+</font> | ||
|[[Image:Imperial_CBD_Design_CBD3.png|110px]] | |[[Image:Imperial_CBD_Design_CBD3.png|110px]] | ||
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
| - | |width="40%"| | + | |width="40%"| [[Image:IC2007_Experiments_Phase1_Protocol1-1.jpg|center|350px]]<br /> |
* '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.1 Results]) | * '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.1 Results]) | ||
* '''Sequencing of Construct''': Completed | * '''Sequencing of Construct''': Completed | ||
| - | |width="40%"| | + | |width="40%"| [[Image:IC_2007_PT7-invitro.png|center|350px]]<br /> |
* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.9|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 Results]) | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.9|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 Results]) | ||
* '''Sequencing of Construct''': Completed | * '''Sequencing of Construct''': Completed | ||
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]] | ||
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in vitro''''': | + | * '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]]) |
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2| | + | * '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.2 Results]) |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status''' | ||
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<center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center> | <center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center> | ||
Latest revision as of 02:35, 27 October 2007
Cell by Date: Implementation
| | |||||||
| Construct Design | Concept 1 | Concept 2 | |||||
Stage 1: Availability 
| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry | Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry. | |||||
| Stage 2: Cloning / Amplification
|  |  | |||||
| Stage 3: Ligation
| Not Required | Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
| |||||
| Stage 4: Purification of Activator Proteins
| Not Required | Not Required | |||||
| Stage 5: Quality Control
|  |  | |||||
| Stage 6: Final Implementation Status | Ready for Testing | Ready for Testing | |||||
| | ||
| Chassis Design | 
| |
| Stage 1: Availability
| Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. |
| Stage 2: Preparation
| Preparation: Done (Protocol) | Preparation: Done (Protocol) |
| Stage 3: Quality Control
| ||
| Stage 4: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |
