Imperial

From 2007.igem.org

(Difference between revisions)
 
(87 intermediate revisions not shown)
Line 1: Line 1:
{{Template:IC07navmenu}}
{{Template:IC07navmenu}}
-
 
<html>
<html>
-
<link rel="stylesheet" href="/igem07/index.php?title=User:Alexander.wong/Stylesheets/mainpage.css&action=raw&ctype=text/css" type="text/css" />
+
<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/mainpage.css&action=raw&ctype=text/css" type="text/css" />
<div id="wrap">
<div id="wrap">
<div id="maincontent">
<div id="maincontent">
-
<!-- Place Team Photo under here -->
+
<!-- Start of Central Content Box -->
-
<img src="http://openwetware.org/images/1/1a/IC07_teamphoto.jpg" width="620px" >
+
 +
<div id="maincol">
-
<!-- Start of Main Text Column -->
+
<div id="OverviewBox">
 +
<h2><b>Welcome!</b></h2>
 +
<div class="contentbox">The IC GEMs team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. Our genuine collective interest in healthcare led to the targetting of <b>health and medicine related applications</b> this year. Together with the vision of an industry, the strategy we adopted for our projects is to use the Engineering Cycle to create reliable Synthetic Biology products where standards and quality control are strictly kept.
 +
                </div>
 +
        </div>
-
+
<div id="FeatureBox">
-
<div id="maincol">
+
<div class="contentbox">
-
<div class="blurb">Our contribution to the synthetic biology community will be the investigation of Cell-Free Systems</div>
+
<h1><b>Main Project</b> - <a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">Infector Detector</a></h1>  
-
<p class="main">The Imperial College iGEM 2007 team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. This year our contribution to the synthetic biology community will be the investigation of Cell-Free Systems, the common platform on which Cell By Date and Infector Detector will be built. The cell-free approach is particularly useful for Synthetic Biology to operate in the medical and food industries. We believe that the characterization of this cell-free chassis will unlock fresh potential in simple constructs. Our project strategy is based on the Engineering Cycle. We are at the final testing and polishing stages of our project, and we will soon be finished!</p> <br>
+
-
                <p class="main">This site is currently under construction. For more information, have a look at our <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2007">OpenWetware site</a>.</p>
+
Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.<i>coli</i>, and output a fluorescent protein as a result. (<a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">more...</a>)
-
</div>
+
<br><br>
-
<!-- End of Main Text Column -->
+
<table style="background-color: #f5facf;" width="590px" align="center" border="0" cellpadding="7px" cellspacing="5">
-
<!-- Start of Central Content Box -->
+
  <tr>
-
+
      <td valign="top" style="text-align:justify;">
 +
          <center><img src="https://static.igem.org/mediawiki/2007/d/d5/IC07_IDnews.png" width="260px"></center>
 +
      </td>
 +
      <td>
 +
          <div id="SummaryBox">
 +
<h2>Main Achievements</h2>
 +
<div class="parabox">
 +
                      <ul>
 +
                      <li>Designed and modelled a device to <b>detect biofilms</b> and <b>prevent urinary catheter infections</b> like MRSA
 +
                      <li>Successfully utilized <b>cell-free chassis</b> for Infector Detector
 +
                      <li>Characterised Infector Detector to <b>detect AHL at ambient temperatures</b>
 +
                      <li><a href="https://2007.igem.org/Imperial/Infector_Detector/F2620_Comparison"><b>Compared system expression</b></a> to <a href="http://partsregistry.org/Part:BBa_F2620">AHL Receiver (BBa_F2620)</a> transfer function.
 +
                      </ul>
 +
                </div>
 +
        </div>
 +
      </td>
 +
  </tr>
 +
</table>
 +
 
 +
</div>
 +
</div> 
 +
 
 +
 
 +
<div id="TechnoBox">
 +
<div class="contentbox">
 +
<h2><b>Foundational technology</b> - <a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">Cell-Free Systems</a></h2>
 +
<br>
 +
<center><img src="https://static.igem.org/mediawiki/2007/b/bb/IC07_CFS_components.png" width="400px"></center>
 +
<table style="background-color: #dff7d6;" width="560px" align="center" border="0" cellpadding="7px" cellspacing="5">
 +
      <td valign="top" style="text-align:justify;">Power your iGEM projects with Cell-Free Systems! Find out what this new chassis has done for us, and what it can do for you! (<a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">more...</a>)<br>
 +
 
 +
</table>
 +
                </div>
 +
</div> 
<div id="FeatureBox">
<div id="FeatureBox">
-
<div class="metainfo">
 
-
<h2><a href="http://...">Cell-Free Systems in the Real World</a></h2>
 
-
</div>
 
<div class="contentbox">
<div class="contentbox">
-
                <table style="text-align: justify; background-color: #f5facf;"><tr>
+
<h1><b>Sub Project</b> - <a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">Cell by Date</a></h1>
-
                    <td style="width: 50%; padding-right: 10px; vertical-align: top;">
+
 
-
<h2>Infector Detector</h2> Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.<i>coli</i>. The system is able to detect very small concentrations of AHL, and outputs a fluorescent protein as a result.
+
Cell by Date will report when ground beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8&deg;C. (<a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">more...</a>)<br>
-
                    </td>
+
 
-
                    <td style="width: 50%; padding-left: 10px; vertical-align: top;">
+
<table style="background-color: #f5facf;" width="560px" align="center" border="0" cellpadding="7px" cellspacing="5">
-
<h2>Cell by Date</h2> Cell by Date will tell you when meat has been out of the fridge for too long. It produces fluorescent protein when it is found in an ambient temperature above a threshold of 10&deg;C. The fluorescent protein accumulates over time, and eventually becomes visible to the naked eye once the system has been exposed to high temperatures for enough time.
+
  <tr>
-
                    </td>
+
      <td valign="center"><img src="https://static.igem.org/mediawiki/2007/7/74/CBDCartoonfront.png" width="220px">
-
                </tr></table>
+
 
 +
      <td valign="top" style="text-align:left;">
 +
          <div id="SummaryBox" style="width: 300px;">
 +
<h2>Main Achievements</h2>
 +
<div class="parabox" style="width: 270px;">
 +
                      <ul>
 +
                      <li>Designed and modelled a device to <b>indicate spoilage of meat</b>/break in the cold chain via the <b>thermal history</b> of the system
 +
                      <li> Utilised project results to <b>characterise cell-free chassis</b>
 +
                      <li> Characterised <a href="http://partsregistry.org/Part:BBa_I13522">pTet-GFP</a> and <a href="http://partsregistry.org/Part:BBa_I719015">pT7-GFP</a> under <b>isothermal conditions</b>
 +
                      </ul>
 +
                </div>
 +
        </div>
 +
      </td>
 +
  </tr>
 +
</table>
</div>
</div>
</div>   
</div>   
-
 
-
<!-- <a href="http://..."><img src="http://openwetware.org/images/3/36/IC07_CFS_cartoon.png" width="240px"></a> -->
 
<!-- End of Central Content Box -->
<!-- End of Central Content Box -->
Line 71: Line 116:
-->
-->
<!-- End of Post-It Box Template -->
<!-- End of Post-It Box Template -->
-
<!-- Add your Post-It Boxes in here -->
+
 
 +
<div class="post-it greenbox">
 +
<h2><b><a href="https://2007.igem.org/Imperial/Team" title="">Meet the Team</a></b></h2>
 +
<br />
 +
<img src="https://static.igem.org/mediawiki/2007/9/97/IC07_teamphoto3.jpg" width="230px">
 +
<br>
 +
</div>
<div class="post-it bluebox">
<div class="post-it bluebox">
-
<h2><a href="https://2007.igem.org/Imperial/News">News</a></h2>
+
<h2><b> <a href="https://2007.igem.org/Imperial/Open_Science_on_OpenWetWare" title="">Open Science on OpenWetWare</a></b></h2>
-
<div id="newscol">
+
  <table style="background-color: #d9f0f8; filter:alpha(opacity=80); -moz-opacity:0.8; font-size: .95em;">
 +
        <tr style="font-weight: bold;">
 +
              <td>We did Open Science!</td>
 +
        </tr><tr>
 +
              <td>
 +
              Our projects were run and organised using OWW, with updates on a day-to-day basis! Click <a href="https://2007.igem.org/Imperial/Open_Science_on_OpenWetWare" title="">here to access our <b>400+ pages of full documentation!</b></a>
 +
              </td></tr>
 +
  </table>
 +
</div>
 +
<div class="post-it pinkbox">
 +
<h2><b>Contributions to Registry</b></h2>
 +
  <table style="background-color: #f0dbcc; filter:alpha(opacity=80); -moz-opacity:0.8; font-size: .95em;">
 +
        <tr style="font-weight: bold;">
 +
              <td>Chassis Additions</td>
 +
        </tr><tr>
 +
              <td>
 +
              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems">Cell-Free Systems</a><br>
 +
              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30">- Homemade <i>E. coli</i> S30</a><br>
 +
          <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30">- Commercial <i>E. coli</i> S30</a><br>
 +
              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30">- Commercial <i>E. coli</i> T7 S30</a><br>
 +
              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle">- Vesicle Encapsulation</a><br>
 +
              </td></tr>
 +
        <tr style="font-weight: bold;">
 +
              <td>Part/Construct Additions</td>
 +
        </tr><tr>
 +
              <td>
 +
              <a href="http://partsregistry.org/Part:BBa_I719005">T7 promoter (BBa_I719005)</a><br>
 +
              <a href="http://partsregistry.org/Part:BBa_I719015">pT7 with GFP reporter (BBa_I719015)</a>
 +
              </td></tr>
 +
        <tr style="font-weight: bold;">
 +
              <td>Part/Construct Characterisation</td>
 +
        </tr><tr>
 +
              <td>
 +
              <a href="http://partsregistry.org/Part:BBa_F2620"> AHL Receiver (BBa_F2620)</a> <br>
 +
              <a href="http://partsregistry.org/Part:BBa_I13522">pTet GFP (BBa_I13522)</a><br>
 +
              <a href="http://partsregistry.org/Part:BBa_J37032">pLux GFP (BBa_J37032)</a><br>
 +
              </td>
 +
        </tr>
 +
  </table>
 +
</div>
 +
<div class="post-it yellowbox">
 +
<h2><b><a href="https://2007.igem.org/Imperial/Fun" title="">Fun with iGEM</a></b></h2>
 +
  <table style="background-color: #f7fbd7; filter:alpha(opacity=80); -moz-opacity:0.8; font-size: .95em;">
 +
      <td> Pictures, Quotes and More!
 +
  </table>
-
<!-- Sample News Entry Item -->
 
-
<!-- Copy, Paste, and edit the following to add a news item:
 
-
 
-
 
-
 
-
<div class="newsentry">
 
-
<div class="newsmeta">
 
-
<h2><a href=""></a>News Item Title</h2>
 
-
<span class="newsmeta-date">26 sep 07</span>
 
</div>
</div>
-
<p>Write your news story here.<a class="newsmeta-more" href="http://.....">more</a></p>
 
-
</div>
 
-
 
+
<div class="post-it whitebox" style="filter:alpha(opacity=80); -moz-opacity:0.8;">
-
 
+
<h2><b><a href="https://2007.igem.org/Imperial/Acknowledgements" title="">Acknowledgements</a></b></h2>
-
 
+
  <table style="background-color: #eeeeee; filter:alpha(opacity=80); -moz-opacity:0.8; font-size: .95em;">
-
-->
+
              <td>
-
<!-- Start of News Entries -->
+
              IC Deputy Rector's Fund<br>
-
 
+
              IC Faculty of Engineering<br>
-
 
+
              IC Faculty of Natural Sciences<br>
-
 
+
              European Commission, Synbiocomm Project<br>
-
<div class="newsentry">
+
              </td>
-
<div class="newsmeta">
+
  </table>
-
<h2><a href=""></a>Lab Notebook now Online</h2>
+
-
<span class="newsmeta-date">09 oct 07</span>  
+
</div>
</div>
-
<p>We are now moving our lab notebook across to this new site. In there, you will find experiment details and most of the results before data analysis. <a href="https://2007.igem.org/Imperial/Wet_Lab/Lab_Notebook">Have a look!</a> You can also read about our goofs and flukes, and first hand reports of what was happening in the labs. If you are looking for more information - particularly project details, data analysis, discussions, and more - you can still find it in our <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2007">OpenWetware site</a>.</p>
 
-
</div>
 
-
 
 +
<!-- End of Side Bar Content -->
</div>
</div>
-
<!-- End of News Entries -->
 
-
</div>
 
-
<div class="post-it greenbox">
+
</div>
-
<h2><a href="" title="">Meet the Team</a></h2>
+
-
                    <table style="background-color: #dff7d6; filter:alpha(opacity=80); -moz-opacity:0.8; font-size: .95em;">
+
<!-- DO NOT REMOVE : Start of StatCounter Code -->
-
                      <tr style="font-weight: bold;">
+
<script type="text/javascript">
-
                          <td>Undergraduates</td>
+
var sc_project=3073879;  
-
                          <td style="text-align: right; padding-left: 5px;">Faculty Advisors</td>
+
var sc_invisible=0;  
-
                      </tr>
+
var sc_partition=33;  
-
                      <tr>
+
var sc_security="e7bc3456";  
-
                          <td>James Chappell</td>
+
</script>
-
                          <td style="text-align: right; padding-left: 5px;">Prof Paul Freemont</td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Lucas Hadjilucas</td>
+
-
                          <td style="text-align: right; padding-left: 5px;">Prof Richard Kitney</td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Jaroslaw Karcz</td>
+
-
                          <td style="text-align: right; padding-left: 5px;"></td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Anthony Lazzaro</td>
+
-
                          <td style="text-align: right; font-weight:bold; padding-left: 5px;">Supervisors</td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Peixuan Pey</td>
+
-
                          <td style="text-align: right; padding-left: 5px;">Vincent Rouilly</td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Maira Tariq</td>
+
-
                          <td style="text-align: right; padding-left: 5px;">Matthieu Bultelle</td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Ben Yi Tew</td>
+
-
                          <td style="text-align: right; padding-left: 5px;">Kirsten Jensen</td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Cheuk Ka Tong</td>
+
-
                          <td style="text-align: right; padding-left: 5px;">Dr. Duo Lu</td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Dirk van Swaay</td>
+
-
                          <td style="text-align: right; padding-left: 5px;"></td>
+
-
                      </tr>
+
-
                      <tr>
+
-
                          <td>Alexander Wong</td>
+
-
                          <td></td>
+
-
                      </tr>
+
-
                    </table>
+
 +
<script type="text/javascript" src="http://www.statcounter.com/counter/counter_xhtml.js"></script><noscript><div class="statcounter"><a class="statcounter" href="http://www.statcounter.com/"><img class="statcounter" src="http://c34.statcounter.com/3073879/0/e7bc3456/0/" alt="blog counter" /></a></div></noscript>
 +
<!-- DO NOT REMOVE : End of StatCounter Code -->
-
</div>
 
-
 
-
<div class="post-it pinkbox">
 
-
<h2><a href="" title="">Fun with iGEM</a></h2>
 
-
</div>
 
-
 
-
<div class="post-it whitebox" style="filter:alpha(opacity=80); -moz-opacity:0.8;">
 
-
<h2><a href="" title="">Acknowledgements</a></h2>
 
-
                <ul style="list-style: none;">
 
-
                  <li><a href="http://www.geneart.com"><img src="http://www.geneart.com/fileadmin/templates/img/logo.gif" width="180"></a> 
 
-
                  <li>IC Deputy Rector's Fund
 
-
                  <li>IC Faculty of Engineering
 
-
                  <li>IC Faculty of Natural Sciences
 
-
                </ul>
 
-
</div>
 
-
 
-
 
-
</div>
 
-
<!-- End of Side Bar Content -->
 
-
 
-
 
-
</div>
 
</html>
</html>

Latest revision as of 03:34, 27 October 2007

Welcome!

The IC GEMs team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. Our genuine collective interest in healthcare led to the targetting of health and medicine related applications this year. Together with the vision of an industry, the strategy we adopted for our projects is to use the Engineering Cycle to create reliable Synthetic Biology products where standards and quality control are strictly kept.

Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

Main Achievements

  • Designed and modelled a device to detect biofilms and prevent urinary catheter infections like MRSA
  • Successfully utilized cell-free chassis for Infector Detector
  • Characterised Infector Detector to detect AHL at ambient temperatures
  • Compared system expression to AHL Receiver (BBa_F2620) transfer function.

Foundational technology - Cell-Free Systems


Power your iGEM projects with Cell-Free Systems! Find out what this new chassis has done for us, and what it can do for you! (more...)

Sub Project - Cell by Date

Cell by Date will report when ground beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8°C. (more...)

Main Achievements

  • Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system
  • Utilised project results to characterise cell-free chassis
  • Characterised pTet-GFP and pT7-GFP under isothermal conditions

Retrieved from "http://2007.igem.org/wiki/index.php/Imperial"