Imperial

From 2007.igem.org

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<h2>Project Overview</h2>
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<h2><b>Welcome!</b></h2>
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<div class="parabox">Our contribution to the synthetic biology community this year will be the investigation of Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built.  
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<div class="contentbox">The IC GEMs team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. Our genuine collective interest in healthcare led to the targetting of <b>health and medicine related applications</b> this year. Together with the vision of an industry, the strategy we adopted for our projects is to use the Engineering Cycle to create reliable Synthetic Biology products where standards and quality control are strictly kept.
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<h2>Main Project - <a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">Infector Detector</a></h2>  
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<h1><b>Main Project</b> - <a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">Infector Detector</a></h1>
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Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.<i>coli</i>, and output a fluorescent protein as a result. (<a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">more...</a>)
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       <td valign="top" style="text-align:justify;">Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.<i>coli</i>, and output a fluorescent protein as a result. (<a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">more...</a>)<br>
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<h2>Main Achievements</h2>
<h2>Main Achievements</h2>
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                       <li>Designed and modelled a device to detect biofilms and potentially prevent diseases like MRSA
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                       <li>Designed and modelled a device to <b>detect biofilms</b> and <b>prevent urinary catheter infections</b> like MRSA
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                       <li>Characterised Infector Detector in terms of various AHL concentrations, at room temperature
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                      <li>Successfully utilized <b>cell-free chassis</b> for Infector Detector
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                       <li>Characterised Infector Detector to <b>detect AHL at ambient temperatures</b>
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                      <li><a href="https://2007.igem.org/Imperial/Infector_Detector/F2620_Comparison"><b>Compared system expression</b></a> to <a href="http://partsregistry.org/Part:BBa_F2620">AHL Receiver (BBa_F2620)</a> transfer function.
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      <td align="right" valign="top" style="text-align: center; background-color: #ffffff;">Image for Specs.<br>SPECIFICATIONS
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      <td style="text-align: center; background-color: #ffffff;">Image for Testing/Validation.<br>TESTING / VALIDATION
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      <td style="text-align: center; background-color: #ffffff;">Image for Design.<br>DESIGN
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      <td style="text-align: center; background-color: #ffffff;">Image for full system.<br>INFECTOR DETECTOR
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      <td style="text-align: center; background-color: #ffffff;">Image for Implementation.<br>IMPLEMENTATION
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      <td style="text-align: center; background-color: #ffffff;">Image for Modelling.<br>MODELLING
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<h2>Foundational technology - <a href="https://2007.igem.org/Imperial/Cell-Free/Introduction">Cell-Free Systems</a></h2>
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<h2><b>Foundational technology</b> - <a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">Cell-Free Systems</a></h2>
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<br>
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<center><img src="https://static.igem.org/mediawiki/2007/b/bb/IC07_CFS_components.png" width="400px"></center>
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      <td valign="top" style="text-align:justify;">Power your iGEM projects with Cell-Free Systems! Find out what this new chassis has done for us, and what it can do for you! (<a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">more...</a>)<br>
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      <td valign="top" style="text-align:justify;">Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification requirements. Click <a href="https://2007.igem.org/Imperial/Cell-Free/Introduction">here</a> for more details!<br>
 
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<h2>Main Achievements</h2>
 
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                      <li>Investigated and characterised the <i>in vitro</i> chassis
 
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                      <li>Characterised the F2620, pTet-GFP and pT7-GFP construct <i>in vitro</i>
 
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<h2>Sub Project - <a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">Cell by Date</a></h2>  
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<h1><b>Sub Project</b> - <a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">Cell by Date</a></h1>
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Cell by Date will report when ground beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8&deg;C. (<a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">more...</a>)<br>
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       <td valign="center"><img src="https://static.igem.org/mediawiki/2007/7/74/CBDCartoonfront.png" width="220px">
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       <td valign="top" style="text-align:justify;">Cell by Date will report when beef has been out of the fridge for too long. It produces fluorescent proteins at ambient temperatures above 8&deg;C. (<a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">more...</a>)<br>
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<h2>Main Achievements</h2>
<h2>Main Achievements</h2>
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                       <ul>
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                       <li>Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system
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                       <li>Designed and modelled a device to <b>indicate spoilage of meat</b>/break in the cold chain via the <b>thermal history</b> of the system
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                      <li> Utilised project results to <b>characterise cell-free chassis</b>
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                      <li> Characterised <a href="http://partsregistry.org/Part:BBa_I13522">pTet-GFP</a> and <a href="http://partsregistry.org/Part:BBa_I719015">pT7-GFP</a> under <b>isothermal conditions</b>
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<h2><b><a href="" title="">Meet the Team</a></b></h2>
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<h2><b><a href="https://2007.igem.org/Imperial/Team" title="">Meet the Team</a></b></h2>
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<br />
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<h2><b> <a href="https://2007.igem.org/Imperial/Open_Science_on_OpenWetWare" title="">Open Science on OpenWetWare</a></b></h2>
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                          <td>Undergraduates</td>
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                          <td style="text-align: right; padding-left: 5px;">Faculty Advisors</td>
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              <td>We did Open Science!</td>
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                          <td>James Chappell</td>
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                          <td style="text-align: right; padding-left: 5px;">Prof Paul Freemont</td>
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              Our projects were run and organised using OWW, with updates on a day-to-day basis! Click <a href="https://2007.igem.org/Imperial/Open_Science_on_OpenWetWare" title="">here to access our <b>400+ pages of full documentation!</b></a>
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                          <td>Lucas Hadjilucas</td>
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</div>
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                          <td style="text-align: right; padding-left: 5px;">Prof Richard Kitney</td>
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                          <td>Jaroslaw Karcz</td>
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                          <td>Anthony Lazzaro</td>
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                          <td>Peixuan Pey</td>
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                          <td style="text-align: right; padding-left: 5px;">Vincent Rouilly</td>
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                          <td>Maira Tariq</td>
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                          <td style="text-align: right; padding-left: 5px;">Matthieu Bultelle</td>
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                          <td>Ben Yi Tew</td>
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                          <td style="text-align: right; padding-left: 5px;">Kirsten Jensen</td>
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                          <td>Cheuk Ka Tong</td>
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                          <td style="text-align: right; padding-left: 5px;">Dr. Duo Lu</td>
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                          <td>Dirk van Swaay</td>
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                          <td>Alexander Wong</td>
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<h2><b>Contributions to Registry</b></h2>
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              <td>Chassis Additions</td>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems">Cell-Free Systems</a><br>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30">- Homemade <i>E. coli</i> S30</a><br>
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          <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30">- Commercial <i>E. coli</i> S30</a><br>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30">- Commercial <i>E. coli</i> T7 S30</a><br>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle">- Vesicle Encapsulation</a><br>
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              </td></tr>
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        <tr style="font-weight: bold;">
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              <td>Part/Construct Additions</td>
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        </tr><tr>
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              <td>
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              <a href="http://partsregistry.org/Part:BBa_I719005">T7 promoter (BBa_I719005)</a><br>
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              <a href="http://partsregistry.org/Part:BBa_I719015">pT7 with GFP reporter (BBa_I719015)</a>
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              </td></tr>
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        <tr style="font-weight: bold;">
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              <td>Part/Construct Characterisation</td>
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        </tr><tr>
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              <td>
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              <a href="http://partsregistry.org/Part:BBa_F2620"> AHL Receiver (BBa_F2620)</a> <br>
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              <a href="http://partsregistry.org/Part:BBa_I13522">pTet GFP (BBa_I13522)</a><br>
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              <a href="http://partsregistry.org/Part:BBa_J37032">pLux GFP (BBa_J37032)</a><br>
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<h2><b><a href="" title="">Fun with iGEM</a></b></h2>
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<h2><b><a href="https://2007.igem.org/Imperial/Fun" title="">Fun with iGEM</a></b></h2>
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  <table style="background-color: #f7fbd7; filter:alpha(opacity=80); -moz-opacity:0.8; font-size: .95em;">
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      <td> Pictures, Quotes and More!
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<h2><b><a href="" title="">Acknowledgements</a></b></h2>
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<h2><b><a href="https://2007.igem.org/Imperial/Acknowledgements" title="">Acknowledgements</a></b></h2>
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               IC Faculty of Engineering<br>
               IC Faculty of Engineering<br>
               IC Faculty of Natural Sciences<br>
               IC Faculty of Natural Sciences<br>
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               European Commission, Synbiocomm Project<br><br>
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               European Commission, Synbiocomm Project<br>
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              <a href="http://www.geneart.com"><img src="http://www.geneart.com/fileadmin/templates/img/logo.gif" width="180"></a>
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Latest revision as of 03:34, 27 October 2007

Welcome!

The IC GEMs team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. Our genuine collective interest in healthcare led to the targetting of health and medicine related applications this year. Together with the vision of an industry, the strategy we adopted for our projects is to use the Engineering Cycle to create reliable Synthetic Biology products where standards and quality control are strictly kept.

Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

Main Achievements

  • Designed and modelled a device to detect biofilms and prevent urinary catheter infections like MRSA
  • Successfully utilized cell-free chassis for Infector Detector
  • Characterised Infector Detector to detect AHL at ambient temperatures
  • Compared system expression to AHL Receiver (BBa_F2620) transfer function.

Foundational technology - Cell-Free Systems


Power your iGEM projects with Cell-Free Systems! Find out what this new chassis has done for us, and what it can do for you! (more...)

Sub Project - Cell by Date

Cell by Date will report when ground beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8°C. (more...)

Main Achievements

  • Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system
  • Utilised project results to characterise cell-free chassis
  • Characterised pTet-GFP and pT7-GFP under isothermal conditions

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