Template:BerkiGEM2007 AustinConstructionFiles
From 2007.igem.org
| Line 23: | Line 23: | ||
[[BerkiGEM2007-AustinConstructionFile23 | 9145-I716073(built): unmutated Di-alpha fusion, biobricked]]<br> | [[BerkiGEM2007-AustinConstructionFile23 | 9145-I716073(built): unmutated Di-alpha fusion, biobricked]]<br> | ||
[[BerkiGEM2007-AustinConstructionFile24 | 9145-I716074: pTrc-(NO SCAR)-rbs lib-beta 1X mutant, providence (Lys82Asp) - rbs lib - unmutated di-alpha- (Best Yield)]]<br> | [[BerkiGEM2007-AustinConstructionFile24 | 9145-I716074: pTrc-(NO SCAR)-rbs lib-beta 1X mutant, providence (Lys82Asp) - rbs lib - unmutated di-alpha- (Best Yield)]]<br> | ||
| - | [[BerkiGEM2007-AustinConstructionFile25 | 9145-I716075: ]]<br> | + | [[BerkiGEM2007-AustinConstructionFile25 | 9145-I716075: pTrc-(NO SCAR)-rbs lib-beta 2X mutant, providence (Lys82Asp) and Presbyterian (Asn108Lys)-rbs lib - unmutated di alpha - (Best all around)]]<br> |
| - | [[BerkiGEM2007-AustinConstructionFile26 | 9145-I716075: ]]<br> | + | [[BerkiGEM2007-AustinConstructionFile26 | 9145-I716075: pTrc-(NO SCAR)-rbs lib-1X mutant beta, presbyterian (Asn108Lys)-rbs lib-2X mutant alpha (L29F and V96W) (Second best all around)]]<br> |
[[BerkiGEM2007-AustinConstructionFile27 | 9145-I716075: ]]<br> | [[BerkiGEM2007-AustinConstructionFile27 | 9145-I716075: ]]<br> | ||
[[BerkiGEM2007-AustinConstructionFile28 | 9145-I716075: ]]<br> | [[BerkiGEM2007-AustinConstructionFile28 | 9145-I716075: ]]<br> | ||
[[BerkiGEM2007-AustinConstructionFile29 | 9145-I716075: ]]<br> | [[BerkiGEM2007-AustinConstructionFile29 | 9145-I716075: ]]<br> | ||
[[BerkiGEM2007-AustinConstructionFile30 | 9145-I716075: ]]<br> | [[BerkiGEM2007-AustinConstructionFile30 | 9145-I716075: ]]<br> | ||
Revision as of 22:47, 30 June 2007
pBca1101-I716051 (built): Kan cassette RBS pool
pBca1101-I716052(built): Cmr cassette RBS pool
9145-I716053(built): alpha HbA in 9145(biobricks 2.0 format)
9145-I716054(built): beta HbA in 9145(biobricks 2.0 format)
9145-I716055(built): AHSP in 9145(biobricks 2.0 format)
9145-I716056(built): -kan-RBS library-alpha HbA-
9145-I716057(built): -CmR-RBS library-beta HbA-
9145-I716058(built): 1X mutant HbA alpha (L29F) AKA: I716053m29
9145-I716059(built): 1X mutant HbA beta (Asn108Lys) AKA: I716054m108
9145-I716060(built): beta-alpha cassette (with RBS libraries)
9145-I716061(built): pTRC-rbs-beta-rbs-alpha
9145-I716062(built): 2X mutant HbA alpha (L29F/V96W) AKA: I716053m29m96
9145-I716063(built): 2X mutant HbA beta (Asn108Lys/Lys82Asp) AKA: I716054m108m82
1103-I716064: -pCon-(no scar site)-rbs-beta-rbs-alpha
9145-I716065(built): 2X mutant -Kan-rbs-alpha Hba (L29F and V96W)
9145-I716066(built): 2X mutant -Cmr-beta Hba (Asn108Lys and Lys82Asp)
9145-I716067(built): 2X mutants -rbs library-beta-rbs library-alpha- (with RBS libraries)
9145-I716068: 2X mutant Di-Alpha fusion, biobricked
9145-I716069(built): 2X mutant -pTRC-rbs-beta-rbs-alpha L29F/V96W and Asn108Lys/Lys82Asp
9145-I716070(built): alpha HbA-TT
9145-I716071: pTrc-(NO SCAR)-rbs lib-beta-rbs lib-alpha
9145-I716072: 1X mutant beta Hba (Lys82Asp "Providence")
9145-I716073(built): unmutated Di-alpha fusion, biobricked
9145-I716074: pTrc-(NO SCAR)-rbs lib-beta 1X mutant, providence (Lys82Asp) - rbs lib - unmutated di-alpha- (Best Yield)
9145-I716075: pTrc-(NO SCAR)-rbs lib-beta 2X mutant, providence (Lys82Asp) and Presbyterian (Asn108Lys)-rbs lib - unmutated di alpha - (Best all around)
9145-I716075: pTrc-(NO SCAR)-rbs lib-1X mutant beta, presbyterian (Asn108Lys)-rbs lib-2X mutant alpha (L29F and V96W) (Second best all around)
9145-I716075:
9145-I716075:
9145-I716075:
9145-I716075:
