Imperial

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===BactoCops - Imperial College iGEM 2007 Team===
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[[image:IC07_cellbydate.png|thumb|right|320px|Cell By Date]]
 
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The Imperial College iGEM 2007 team consists of ten undergraduate students, as well as a number of graduate students and faculty supervisors. This year, we are engineering '''Bactocops''', biological systems that report the presence of nasty bacteria. Under the Cell-Free Intelligence (CFI), we have two divisions. First, a surveillance team called '''Cell By Date''' that determines when food is spoilt more accurately than printed sell by dates. It exploits the thermal dependence of the rate of expression of a simple reporter system. The second division consists of an undercover team - '''Infector Detector''', which detects biofilms that are antibiotic-resistant and a major source of infection in hospitals. This system makes use of Lux quorum sensing to eavesdrop on the communication between biofilm-forming bacteria.
 
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[[image:IC07_biofilm.png|thumb|left|300px|Infector Detector]]
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<h2><b>Welcome!</b></h2>
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<div class="contentbox">The IC GEMs team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. Our genuine collective interest in healthcare led to the targetting of <b>health and medicine related applications</b> this year. Together with the vision of an industry, the strategy we adopted for our projects is to use the Engineering Cycle to create reliable Synthetic Biology products where standards and quality control are strictly kept.
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Our contributions to the synthetic biology community will be the characterization of '''Cell-Free Chassis''', the common platform on which Cell By Date and Infector Detector will be built. The cell-free approach is particularly useful for Bactocops to operate in the food and medical industries. This is because living, replicating engineered bacteria pose major health risks. We believe this new chassis will unlock fresh potential in simple constructs. Our project strategy is based on the '''Engineering Cycle''', of which we have completed specification and design of the systems. We are starting on modelling and implementation and we aim to test our final constructs in the new chassis. By the end of the summer, the Bactocops will be combat-ready.
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<h1><b>Main Project</b> - <a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">Infector Detector</a></h1>
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Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.<i>coli</i>, and output a fluorescent protein as a result. (<a href="https://2007.igem.org/Imperial/Infector_Detector/Introduction">more...</a>)
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Have a look at our project on [http://openwetware.org/wiki/IGEM:IMPERIAL/2007 OpenWetWare].
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<h2>Main Achievements</h2>
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                      <li>Designed and modelled a device to <b>detect biofilms</b> and <b>prevent urinary catheter infections</b> like MRSA
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                      <li>Successfully utilized <b>cell-free chassis</b> for Infector Detector
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                      <li>Characterised Infector Detector to <b>detect AHL at ambient temperatures</b>
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                      <li><a href="https://2007.igem.org/Imperial/Infector_Detector/F2620_Comparison"><b>Compared system expression</b></a> to <a href="http://partsregistry.org/Part:BBa_F2620">AHL Receiver (BBa_F2620)</a> transfer function.
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<h2><b>Foundational technology</b> - <a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">Cell-Free Systems</a></h2>
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      <td valign="top" style="text-align:justify;">Power your iGEM projects with Cell-Free Systems! Find out what this new chassis has done for us, and what it can do for you! (<a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">more...</a>)<br>
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<h1><b>Sub Project</b> - <a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">Cell by Date</a></h1>
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Cell by Date will report when ground beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8&deg;C. (<a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">more...</a>)<br>
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          <div id="SummaryBox" style="width: 300px;">
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<h2>Main Achievements</h2>
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                      <ul>
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                      <li>Designed and modelled a device to <b>indicate spoilage of meat</b>/break in the cold chain via the <b>thermal history</b> of the system
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                      <li> Utilised project results to <b>characterise cell-free chassis</b>
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                      <li> Characterised <a href="http://partsregistry.org/Part:BBa_I13522">pTet-GFP</a> and <a href="http://partsregistry.org/Part:BBa_I719015">pT7-GFP</a> under <b>isothermal conditions</b>
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<a style="float:right;" href="http://...">Learn More</a>
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<h2><b><a href="https://2007.igem.org/Imperial/Team" title="">Meet the Team</a></b></h2>
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<img src="https://static.igem.org/mediawiki/2007/9/97/IC07_teamphoto3.jpg" width="230px">
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<h2><b> <a href="https://2007.igem.org/Imperial/Open_Science_on_OpenWetWare" title="">Open Science on OpenWetWare</a></b></h2>
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              <td>We did Open Science!</td>
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              Our projects were run and organised using OWW, with updates on a day-to-day basis! Click <a href="https://2007.igem.org/Imperial/Open_Science_on_OpenWetWare" title="">here to access our <b>400+ pages of full documentation!</b></a>
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<h2><b>Contributions to Registry</b></h2>
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              <td>Chassis Additions</td>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems">Cell-Free Systems</a><br>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30">- Homemade <i>E. coli</i> S30</a><br>
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          <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30">- Commercial <i>E. coli</i> S30</a><br>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30">- Commercial <i>E. coli</i> T7 S30</a><br>
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              <a href="http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle">- Vesicle Encapsulation</a><br>
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              <td>Part/Construct Additions</td>
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              <a href="http://partsregistry.org/Part:BBa_I719005">T7 promoter (BBa_I719005)</a><br>
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              <a href="http://partsregistry.org/Part:BBa_I719015">pT7 with GFP reporter (BBa_I719015)</a>
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              <td>Part/Construct Characterisation</td>
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              <a href="http://partsregistry.org/Part:BBa_F2620"> AHL Receiver (BBa_F2620)</a> <br>
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              <a href="http://partsregistry.org/Part:BBa_I13522">pTet GFP (BBa_I13522)</a><br>
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              <a href="http://partsregistry.org/Part:BBa_J37032">pLux GFP (BBa_J37032)</a><br>
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<h2><b><a href="https://2007.igem.org/Imperial/Fun" title="">Fun with iGEM</a></b></h2>
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<h2><b><a href="https://2007.igem.org/Imperial/Acknowledgements" title="">Acknowledgements</a></b></h2>
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              IC Deputy Rector's Fund<br>
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              IC Faculty of Engineering<br>
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              IC Faculty of Natural Sciences<br>
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              European Commission, Synbiocomm Project<br>
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Latest revision as of 03:34, 27 October 2007

Welcome!

The IC GEMs team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. Our genuine collective interest in healthcare led to the targetting of health and medicine related applications this year. Together with the vision of an industry, the strategy we adopted for our projects is to use the Engineering Cycle to create reliable Synthetic Biology products where standards and quality control are strictly kept.

Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

Main Achievements

  • Designed and modelled a device to detect biofilms and prevent urinary catheter infections like MRSA
  • Successfully utilized cell-free chassis for Infector Detector
  • Characterised Infector Detector to detect AHL at ambient temperatures
  • Compared system expression to AHL Receiver (BBa_F2620) transfer function.

Foundational technology - Cell-Free Systems


Power your iGEM projects with Cell-Free Systems! Find out what this new chassis has done for us, and what it can do for you! (more...)

Sub Project - Cell by Date

Cell by Date will report when ground beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8°C. (more...)

Main Achievements

  • Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system
  • Utilised project results to characterise cell-free chassis
  • Characterised pTet-GFP and pT7-GFP under isothermal conditions

Retrieved from "http://2007.igem.org/wiki/index.php/Imperial"