Imperial/Cell-Free/Contribution

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== ''In vitro'' Expression ==
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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Whatis" title=""><span>What is Cell-Free?</span></a></li>
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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Comparison" title=""><span>Advantages of CFS</span></a></li>
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    <li><a class="current" href="https://2007.igem.org/Imperial/Cell-Free/Contribution" title=""><span>Our Contributions</span></a></li>
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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Characterisation" title=""><span>Characterisation</span></a></li>
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    <li><a href="https://2007.igem.org/Imperial/Cell-Free/Applications" title=""><span>CFS Applications</span></a></li>
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= Cell-Free: Our Contribution =
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== ''In vitro'' Expression ==
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*Attempted to make S30 ''E. coli'' cell extract and feeding solution
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*Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
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*Successfully used homemade S30 ''E. coli'' cell extract with commercial feeding solution for expression of GFP
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*Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 ''E. coli'' cell extract and feeding solution
== Vesicles Formation ==
== Vesicles Formation ==
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*Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
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*Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract
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*Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
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*Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
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== Documentation on Registry of Parts ==
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Documented our findings with the following Registry Additions:
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[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade ''E. coli'' S30]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial ''E. coli'' S30]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial ''E. coli'' T7 S30]
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* [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]
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== Documentation on Registry of Parts ==
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<center> [https://2007.igem.org/Imperial/Cell-Free/Comparison << Advantages of CFS] | Our Contribution | [https://2007.igem.org/Imperial/Cell-Free/Characterisation Characterisation >>]
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Latest revision as of 19:55, 26 October 2007



Cell-Free: Our Contribution

In vitro Expression

  • Attempted to make S30 E. coli cell extract and feeding solution
  • Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
  • Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
  • Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution


Vesicles Formation

  • Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
  • Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
  • Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
  • Attempting to find suitable pore proteins to prolong vesicle and expression lifespan


Documentation on Registry of Parts

Documented our findings with the following Registry Additions:

[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]

  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade E. coli S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial E. coli S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial E. coli T7 S30]
  • [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]



<< Advantages of CFS | Our Contribution | Characterisation >>