Latest revision as of 02:35, 27 October 2007

Cell by Date: Implementation

DNA Construct Implementation

Construct Design

Concept 1

Concept 2

Stage 1: Availability

Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry

Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.

Stage 2: Cloning / Amplification

BBa_I13522 - Lane R2 (937bp + 2079bp vector)

BBa_E7104 - Lane 11 (Missing 826bp)

Stage 3: Ligation

Not Required

Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]

+

=

Stage 4: Purification of Activator Proteins

Not Required

Stage 5: Quality Control

IC2007 Experiments Phase1 Protocol1-1.jpg

Testing in vivo: Passed (Protocols and Results)
Sequencing of Construct: Completed

Testing in vitro: Passed (Protocols and Results)
Sequencing of Construct: Completed

Stage 6: Final Implementation Status

Ready for Testing

Chassis Construct Implementation
Chassis Design
Stage 1: Availability	Possibility of making Homemade S30 Cell Extract.	Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation	Preparation: Done (Protocol)	Preparation: Done (Protocol)
Stage 3: Quality Control	Testing in vitro: Failed (Protocol and Results)	Testing in vitro: Passed (Protocol and Results)
Stage 4: Final Implementation Status	Aborted. Expression capacity of chassis was low.	Ready for Testing

<< Modelling | Implementation | Testing/Validation >>

@@ Line 1: / Line 1: @@
 {{Template:IC07navmenu}}
+<br clear="all">
+<html>
+<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" />
+<div id="tabs">
+  <ul>
+    <li><a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction" title=""><span>Introduction</span></a></li>
+    <li><a href="https://2007.igem.org/Imperial/Cell_by_Date/Specification" title=""><span>Specifications</span></a></li>
+    <li><a href="https://2007.igem.org/Imperial/Cell_by_Date/Design" title=""><span>Design</span></a></li>
+    <li><a href="https://2007.igem.org/Imperial/Cell_by_Date/Modelling" title=""><span>Modelling</span></a></li>
+    <li><a class="current" href="https://2007.igem.org/Imperial/Cell_by_Date/Implementation" title=""><span>Implementation</span></a></li>
+    <li><a href="https://2007.igem.org/Imperial/Cell_by_Date/Testing" title=""><span>Testing</span></a></li>
+    <li><a href="https://2007.igem.org/Imperial/Cell_by_Date/Conclusion" title=""><span>Conclusion</span></a></li>
+  </ul>
+</div>
+<hr />
+<br clear="all">
+</html>
 __NOTOC__
+= Cell by Date: Implementation =
-== Constructs ==
+{|border=1 width=100% align="center" cellpadding="10px"
+|-
+|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center>
+|- align="left"
+|wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design'''
+|width="40%"| '''Concept 1'''<br />[[Image:IC07_design_CBDcon1.png|center|370px]]
+|width="40%"| '''Concept 2'''<br />[[Image:IC07_design_CBDcon2.png|center|370px]]
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry
+|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]]
+|width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]]
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"| Not Required
+|width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
+<br>
+{| width="80%"
+|-
+|[[Image:Imperial_CBD_Design_CBD4.png|37px]]
+|<font size="+3">+</font>
+|[[Image:Imperial_CBD_Design_CBD3.png|110px]]
+|<font size="+3">=</font>
+|[[Image:Imperial CBD Design CBD2.2.png|130px]]
+|}
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"| Not Required
+|width="40%"| Not Required
+|-
+|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"| [[Image:IC2007_Experiments_Phase1_Protocol1-1.jpg|center|350px]]<br />
+* '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.1 Results])
+* '''Sequencing of Construct''': Completed
+|width="40%"| [[Image:IC_2007_PT7-invitro.png|center|350px]]<br />
+* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.9|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 Results])
+* '''Sequencing of Construct''': Completed
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
+|width="40%" style="background-color:#b8efb0"| Ready for Testing
+|width="40%" style="background-color:#b8efb0"| Ready for Testing
+|}
+<br clear="all">
+{|border=1 width=100% align="center" cellpadding="10px"
+|-
+|colspan="3" style="background-color:#eeeeff"| <center>'''Chassis Construct Implementation'''</center>
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Chassis Design'''
+|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"| Possibility of making Homemade S30 Cell Extract.
+|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
+|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
+|-
+|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
+|width="40%"|
+* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
+|width="40%"|
+* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.2 Results])
+|- align="center"
+|width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status'''
+|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
+|width="40%" style="background-color:#b8efb0"| Ready for Testing
+|}
+<br clear="all">
-== Chassis ==
-cell extract preparation protocols.
+<center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center>

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Imperial/Cell by Date/Implementation

From 2007.igem.org

Latest revision as of 02:35, 27 October 2007

Cell by Date: Implementation