Imperial/Wet Lab/Protocols

From 2007.igem.org

< Imperial | Wet Lab(Difference between revisions)
 
(69 intermediate revisions not shown)
Line 1: Line 1:
 +
<br clear="all">
 +
{{Template:IC07navmenu}}
{{Template:IC07navmenu}}
__NOTOC__
__NOTOC__
 +
<br clear="all">
= Wet Lab: Protocols =
= Wet Lab: Protocols =
Below is a databank of all the protocols that have been used in our projects.  
Below is a databank of all the protocols that have been used in our projects.  
Line 8: Line 11:
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
{| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
|- style="background:#aabadd; color:Black" align="center"  
|- style="background:#aabadd; color:Black" align="center"  
-
|| <font size=3>'''Fabrication Protocols'''</font>|| <font size=3>'''Testing Protocols'''</font>
+
||<font size=2>'''General Experiments'''</font>||<font size=2>'''Testing Experiments'''</font>
|- style="background:#d5dcef; color:Black" valign="top"
|- style="background:#d5dcef; color:Black" valign="top"
|
|
-
<font size=3>'''Construct Protocols'''</font> <br>
+
<font size=2><font color=green>'''Protein Purification'''</font> <br>
-
* [[Imperial/Wet_Lab/Protocols/DNA1.1|Cloning of DNA- MiniPrep]]
+
* ''Purification of LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.5|Results]]  
-
* [[Imperial/Wet_Lab/Protocols/DNA1.2|Cloning of DNA- MidiPrep]]
+
* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.6|Results]]
-
* [[Imperial/Wet_Lab/Protocols/DNA1.3|Cloning of DNA- MaxiPrep]]
+
-
 
+
<br>
<br>
-
<font size=3>'''Protein Purification'''</font> <br>
+
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
-
* [[Imperial/Wet_Lab/Protocols/Prot1.5|Purification of LuxR]]
+
* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.1|Results]]<br>
-
* [[Imperial/Wet_Lab/Protocols/Prot1.7|Testing of Purified LuxR]]
+
<font size=2><font color=green>'''Calibration Curve'''</font> <br>
-
* [[Imperial/Wet_Lab/Protocols/Prot1.6|Purification of GFPmut3b]]
+
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.3|Results]]
-
* [[Imperial/Wet_Lab/Protocols/Prot1.9|GFPmut3b Calibration Curve (Wrong, this is the recombinant GFP]]
+
* ''Degradation Curve for GFPmut3b'':[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.4|Results]]
-
 
+
<br>
<br>
-
<font size=3>'''Fluorometer Testing'''</font> <br>
+
<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
-
 
+
* ''Reaction Mixture of Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
-
* [[Imperial/Wet_Lab/Protocols/Prot1.1|Optimsation of Fluorometer]]
+
* ''Reaction Mixture of Homemade S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.4|Protocol]]
-
* [[Imperial/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation?? Wrong.]]
+
* ''Recipe for S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
-
* [[Imperial/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]]
+
* ''Recipe for S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
-
* [[Imperial/Wet_Lab/Protocols/Prot1.3|Calibration Curve for DsRed express]]
+
* ''Testing of Homemade S30 Cell Extract with ID construct '':[[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1|Results]]<br>
-
* [[Imperial/Wet_Lab/Protocols/Prot1.4|Degradation Curve (For GFP? Non-existent)]]
+
* ''Vesicles Formation'': [[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
-
* [[Imperial/Wet_Lab/Protocols/Prot1.8|DNA concentration experiments]]
+
<br>
-
 
+
|
 +
<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
 +
*''pT7 in vitro:'' [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.9|Results]]
<br>
<br>
-
<font size=3>'''Chassis Protocols'''</font> <br>
 
-
'''''E. coli'' Extracts'''<br>
 
-
* [[Imperial/Wet_Lab/Protocols/CE1.1|Preparation of S30 ''E. coli'' extract]]
 
-
* [[Imperial/Wet_Lab/Protocols/CE1.2|Preparation of S12 ''E. coli'' extract]]
 
-
* [[Imperial/Wet_Lab/Protocols/Protocols/CE1.3|Preparation of reaction mixture]]
 
-
 
-
'''Vesicles Formation'''<br>
 
-
* [[Imperial/Wet_Lab/Protocols/VF1.1|Vesicles Formation]]
 
-
* 2
 
-
 
-
|
 
-
<font size=3>'''Cell by Date'''</font> <br>
 
-
'''Implementation'''<br>
 
-
* [[Imperial/Wet_Lab/Protocols/CBD1.1|Initial Testing for DNA constructs of CBD In Vivo]]
 
-
* [[Imperial/Wet_Lab/Protocols/CBD1.2|Initial Testing for DNA constructs of CBD In Vitro (Chassis)]]
 
-
 
-
 
-
'''Testing/Validation'''<br>
 
-
* [[Imperial/Wet_Lab/Protocols/CBD2.1|Operating Temperature Range (Initial Tests)]]
 
-
* [[Imperial/Wet_Lab/Protocols/CBD2.2|Operating Temperature Range (Refined Tests)]]
 
-
 
 +
<font size=2>'''Cell by Date'''</font> <br>
 +
<font color=green>'''Implementation'''</font><br>
 +
* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.1|Results]]
 +
* ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.2|Results]]
 +
<font color=green>'''Testing/Validation<br>'''</font>
 +
*''Testing DNA Concentration in vitro'': [[Imperial/Wet_Lab/Protocols/CBD1.3|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
 +
* ''Isothermal experiments'': [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results]]
 +
*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.3|Results]]
 +
*''Packaging experments:'' [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.1|Results]]
<br>
<br>
-
<font size=3>'''Infector Detector'''</font> <br>
 
-
'''Implementation'''<br>
 
-
* [[Imperial/Wet_Lab/Protocols/ID1.1|Initial Testing for DNA constructs of ID In Vivo]]
 
-
* [[Imperial/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro (Chassis)]]
 
 +
<font size=2>'''Infector Detector'''</font> <br>
 +
<font color=green>'''Implementation<br>'''</font>
 +
*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.1|Results]]
 +
* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.2|Results]]
 +
<font color=green>'''Testing/Validation<br>'''</font>
 +
*''Testing of DNA concentration'' : [[Imperial/Wet_Lab/Protocols/ID2.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID2.1|Results]]
 +
* ''Testing of Purified LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.7|Results]]
 +
* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID3.1|Results]]
 +
|}
 +
<br clear="all">
-
'''Testing/Validation'''<br>
 
-
* [[Imperial/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]]
 
-
* [[Imperial/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]]
 
-
* [[Imperial/Wet_Lab/Protocols/ID3.1|Final AHL Testing (Standardized)]]
 
-
 
+
<center>| [[Imperial/Wet_Lab| Wet Lab >>]]</center>
-
|}
+
-
<br clear="all">
+

Latest revision as of 02:15, 27 October 2007




Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols

  • Reaction Mixture of Commercially available S30 Cell Extract :Protocol
  • Reaction Mixture of Homemade S30 Cell Extract :Protocol
  • Recipe for S30 Cell Extract :Protocol
  • Recipe for S12 Cell Extract :Protocol
  • Testing of Homemade S30 Cell Extract with ID construct :Protocol and Results
  • Vesicles Formation: Protocol


pT7 Characterisation


Cell by Date
Implementation

Testing/Validation


Infector Detector
Implementation

Testing/Validation



| Wet Lab >>

Retrieved from "http://2007.igem.org/wiki/index.php/Imperial/Wet_Lab/Protocols"