Imperial/Cell by Date/Implementation

From 2007.igem.org

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{{Template:IC07navmenu}}
{{Template:IC07navmenu}}
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<br clear="all">
<html>
<html>
<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" />
<link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" />
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|- align="left"
|- align="left"
|wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design'''
|wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design'''
-
|width="40%"| '''Concept 1'''<br />[[Image:Imperial CBD Design CBD1.png|center]]
+
|width="40%"| '''Concept 1'''<br />[[Image:IC07_design_CBDcon1.png|center|370px]]
-
|width="40%"| '''Concept 2'''<br />[[Image:Imperial CBD Design CBD2.png|center]]
+
|width="40%"| '''Concept 2'''<br />[[Image:IC07_design_CBDcon2.png|center|370px]]
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry
|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry
|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]]
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_I13522 - Lane R2 (937bp + 2079bp vector)]]
|width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]]
|width="40%"| [[Image:IC07_CBD1-cloning.png|thumb|center|290px| BBa_E7104 - Lane 11 (Missing 826bp)]]
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
|width="40%"| Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
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{| width="80%"
{| width="80%"
|-
|-
-
|[[Image:Imperial_CBD_Design_CBD4.png|30px]]
+
|[[Image:Imperial_CBD_Design_CBD4.png|37px]]
|<font size="+3">+</font>
|<font size="+3">+</font>
|[[Image:Imperial_CBD_Design_CBD3.png|110px]]
|[[Image:Imperial_CBD_Design_CBD3.png|110px]]
|<font size="+3">=</font>
|<font size="+3">=</font>
-
|[[Image:Imperial CBD Design CBD2.png|130px]]
+
|[[Image:Imperial CBD Design CBD2.2.png|130px]]
|}
|}
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Not Required
|width="40%"| Not Required
|-  
|-  
-
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
+
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
-
|width="40%"|  
+
|width="40%"| [[Image:IC2007_Experiments_Phase1_Protocol1-1.jpg|center|350px]]<br />
-
* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
+
* '''Testing ''in vivo''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.1 Results])
-
* '''Sequencing of Construct''': Pending
+
* '''Sequencing of Construct''': Completed
-
|width="40%"|  
+
|width="40%"| [[Image:IC_2007_PT7-invitro.png|center|350px]]<br />
-
* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results])
+
* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/Prot1.9|Protocols]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/Res1.9 Results])
-
* '''Sequencing of Construct''': Pending
+
* '''Sequencing of Construct''': Completed
|- align="center"
|- align="center"
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
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|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
|colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]]
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| Possibility of making Homemade S30 Cell Extract.
|width="40%"| Possibility of making Homemade S30 Cell Extract.
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
|width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
|width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]])
|-  
|-  
-
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control'''
+
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' <br /> <br> [[Image:Icegems-Devcycle-downarrow.png|30px]]
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vitro''''': Done (Protocol and Results)
+
* '''Testing ''in vitro''''': Failed ([[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1 |Results]])
|width="40%"|  
|width="40%"|  
-
* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results])
+
* '''Testing ''in vitro''''': Passed ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocol]] and [https://2007.igem.org/Imperial/Wet_Lab/Results/CBD1.2 Results])
|- align="center"
|- align="center"
-
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
+
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Final Implementation Status'''
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low.
|width="40%" style="background-color:#b8efb0"| Ready for Testing
|width="40%" style="background-color:#b8efb0"| Ready for Testing
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-
== Navigation==
+
 
<center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center>
<center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center>

Latest revision as of 02:35, 27 October 2007



Cell by Date: Implementation

DNA Construct Implementation
Construct Design Concept 1
IC07 design CBDcon1.png
 Concept 2
IC07 design CBDcon2.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png 		Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
Stage 2: Cloning / Amplification

Icegems-Devcycle-downarrow.png
BBa_I13522 - Lane R2 (937bp + 2079bp vector)
BBa_E7104 - Lane 11 (Missing 826bp)
Stage 3: Ligation

Icegems-Devcycle-downarrow.png 		Not Required Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.2.png
Stage 4: Purification of Activator Proteins

Icegems-Devcycle-downarrow.png 		Not Required Not Required
Stage 5: Quality Control

Icegems-Devcycle-downarrow.png
IC2007 Experiments Phase1 Protocol1-1.jpg

  • Testing in vivo: Passed (Protocols and Results)
  • Sequencing of Construct: Completed
IC 2007 PT7-invitro.png

  • Testing in vitro: Passed (Protocols and Results)
  • Sequencing of Construct: Completed
Stage 6: Final Implementation Status Ready for Testing Ready for Testing



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability

Icegems-Devcycle-downarrow.png 		Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation

Icegems-Devcycle-downarrow.png 		Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control

Icegems-Devcycle-downarrow.png
Stage 4: Final Implementation Status Aborted.
Expression capacity of chassis was low. 		Ready for Testing



<< Modelling | Implementation | Testing/Validation >>
Retrieved from "http://2007.igem.org/wiki/index.php/Imperial/Cell_by_Date/Implementation"