Latest revision as of 02:15, 27 October 2007

Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General Experiments

Testing Experiments

Protein Purification

Purification of LuxR: Protocol and Results
Purification of GFPmut3b: Protocol and Results

Fluorometer Testing

Optimisation of Fluorometer: Protocol and Results

Calibration Curve

Calibration Curve for GFPmut3b: Protocol and Results
Degradation Curve for GFPmut3b:Protocol and Results

Chassis Protocols

Reaction Mixture of Commercially available S30 Cell Extract :Protocol
Reaction Mixture of Homemade S30 Cell Extract :Protocol
Recipe for S30 Cell Extract :Protocol
Recipe for S12 Cell Extract :Protocol
Testing of Homemade S30 Cell Extract with ID construct :Protocol and Results
Vesicles Formation: Protocol

pT7 Characterisation

pT7 in vitro: Protocol and Results

Cell by Date
Implementation

In Vivo Testing: Protocols and Results
Initial In vitro Testing: Protocols and Results

Testing/Validation

Testing DNA Concentration in vitro: Protocols and Results
Isothermal experiments: Protocols and Results
Temperature Steps: Protocols and Results
Packaging experments: Protocols and Results

Infector Detector
Implementation

In vivo Testing: Protocol and Results
Initial In Vitro Testing: Protocol and Results

Testing/Validation

Testing of DNA concentration : Protocol and Results
Testing of Purified LuxR: Protocol and Results
Characterization in vitro - AHL titration : Protocol and Results

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 = Wet Lab: Protocols =
 Below is a databank of all the protocols that have been used in our projects.
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 <font size=2><font color=green>'''Calibration Curve'''</font> <br>
 * ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.3|Results]]
-* ''Degradation Curve for GFPmut3b'':[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.4|Results]][http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.4 Results]
+* ''Degradation Curve for GFPmut3b'':[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.4|Results]]
 <br>
 <font size=2><font color=green>'''Chassis Protocols'''</font> <br>
-* ''Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
+* ''Reaction Mixture of Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
-* ''Preparation S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
+* ''Reaction Mixture of Homemade S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.4|Protocol]]
-* ''Preparation S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
+* ''Recipe for S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
-* ''Vesicles Formation'':[[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
+* ''Recipe for S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
+* ''Testing of Homemade S30 Cell Extract with ID construct '':[[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1|Results]]<br>
+* ''Vesicles Formation'': [[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
 <br>
 |
 <font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
-*''pT7 in vitro:'' [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/Protocols/Prot1.9 Protocols] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.9 Results]
+*''pT7 in vitro:'' [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.9|Results]]
 <br>
 <font size=2>'''Cell by Date'''</font> <br>
 <font color=green>'''Implementation'''</font><br>
-* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results]
+* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.1|Results]]
-* ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results]
+* ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.2|Results]]
 <font color=green>'''Testing/Validation<br>'''</font>
-*''Testing DNA Concentration in vitro'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD4 Results]
+*''Testing DNA Concentration in vitro'': [[Imperial/Wet_Lab/Protocols/CBD1.3|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
-* ''Operating Temperature Range'': [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.2 Results 1] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
+* ''Isothermal experiments'': [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results]]
-*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]
+*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.3|Results]]
+*''Packaging experments:'' [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.1|Results]]
 <br>
-<font size=2>'''Infecter Detector'''</font> <br>
+<font size=2>'''Infector Detector'''</font> <br>
 <font color=green>'''Implementation<br>'''</font>
-*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]
+*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.1|Results]]
-* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]
+* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.2|Results]]
 <font color=green>'''Testing/Validation<br>'''</font>
-*''Testing of DNA concentration'' : [[Imperial/Wet_Lab/Protocols/ID2.1|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res2.1 Results]
+*''Testing of DNA concentration'' : [[Imperial/Wet_Lab/Protocols/ID2.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID2.1|Results]]
-* ''Testing of Purified LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]
+* ''Testing of Purified LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.7|Results]]
-* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 Results]
+* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID3.1|Results]]
 |}
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+<center>| [[Imperial/Wet_Lab| Wet Lab >>]]</center>

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Imperial/Wet Lab/Protocols

From 2007.igem.org

Latest revision as of 02:15, 27 October 2007

Wet Lab: Protocols