Imperial/Cell-Free/Contribution
From 2007.igem.org
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| - | + | = Cell-Free: Our Contribution = | |
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| + | == ''In vitro'' Expression == | ||
*Attempted to make S30 ''E. coli'' cell extract and feeding solution | *Attempted to make S30 ''E. coli'' cell extract and feeding solution | ||
*Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP | *Successfully used commercial S30 ''E. coli'' cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP | ||
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| - | + | == Vesicles Formation == | |
*Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer | *Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer | ||
*Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract | *Successfully formed vesicles encapsulating GFP in homemade S30 ''E. coli'' cell extract | ||
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| - | + | == Documentation on Registry of Parts == | |
Documented our findings with the following Registry Additions: | Documented our findings with the following Registry Additions: | ||
Latest revision as of 19:55, 26 October 2007
Cell-Free: Our Contribution
In vitro Expression
- Attempted to make S30 E. coli cell extract and feeding solution
- Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
- Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
- Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution
Vesicles Formation
- Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
- Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
- Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
- Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
Documentation on Registry of Parts
Documented our findings with the following Registry Additions:
[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade E. coli S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial E. coli S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial E. coli T7 S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]
