Imperial

From 2007.igem.org

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<h2>Project Overview</h2>
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<h2>Our Approach</h2>
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<div class="contentbox">Our contribution to the synthetic biology community this year will be the investigation of Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built.  
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<div class="contentbox">With the vision of an industry, the strategy for our projects this year is to use the Engineering Cycle to create reliable Synthetic Biology  products where standards and quality control are strictly kept.
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       <td valign="top" style="text-align:justify;">Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification requirements. Click <a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">here</a> for more details!<br>
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       <td valign="top" style="text-align:justify;">Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification needs. Click <a href="https://2007.igem.org/Imperial/Cell-Free/Whatis">here</a> for more details!<br>
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       <td valign="center"><img src="https://static.igem.org/mediawiki/2007/thumb/9/96/IC07_cellbydate.png/340px-IC07_cellbydate.png" width="200px">
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       <td valign="top" style="text-align:justify;">Cell by Date will report when beef has been out of the fridge for too long. It produces fluorescent proteins at ambient temperatures above 8&deg;C. (<a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">more...</a>)<br>
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       <td valign="top" style="text-align:justify;">Cell by Date will report when minced beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8&deg;C. (<a href="https://2007.igem.org/Imperial/Cell_by_Date/Introduction">more...</a>)<br>
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Revision as of 02:40, 20 October 2007

Our Approach

With the vision of an industry, the strategy for our projects this year is to use the Engineering Cycle to create reliable Synthetic Biology products where standards and quality control are strictly kept.

Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

Main Achievements

  • Designed and modelled a device to detect biofilms and potentially prevent diseases like MRSA
  • Characterised Infector Detector in terms of various AHL concentrations, at room temperature

Engineering Development Cycle



 Image for Specs.
SPECIFICATIONS
Image for Testing/Validation.
TESTING / VALIDATION 		Image for full system.
 Image for Design.
DESIGN

Image for full system.
INFECTOR DETECTOR
Image for Implementation.
IMPLEMENTATION
Image for Modelling.
MODELLING

Foundational technology - Cell-Free Systems

Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification needs. Click here for more details!

Main Achievements

  • Investigated and characterised the in vitro chassis
  • Characterised the F2620, pTet-GFP and pT7-GFP construct in vitro

Sub Project - Cell by Date

Cell by Date will report when minced beef has been out of the cold chain for too long. It produces fluorescent proteins at ambient temperatures above 8°C. (more...)

Main Achievements

  • Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system

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