Imperial/Cell-Free/Comparison
From 2007.igem.org
(Difference between revisions)
(→''In Vivo'' vs. ''In Vitro'' Systems) |
|||
| Line 1: | Line 1: | ||
{{Template:IC07navmenu}} | {{Template:IC07navmenu}} | ||
__NOTOC__ | __NOTOC__ | ||
| + | <html> | ||
| + | <link rel="stylesheet" href="/igem07/index.php?title=User:Dirkvs/Stylesheets/IC07persist.css&action=raw&ctype=text/css" type="text/css" /> | ||
| + | |||
| + | <div id="tabs"> | ||
| + | <ul> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Whatis" title=""><span>What is Cell-Free?</span></a></li> | ||
| + | <li><a class="current" href="https://2007.igem.org/Imperial/Cell-Free/Comparison" title=""><span>Advantages of CFS</span></a></li> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Contribution" title=""><span>Our Contributions</span></a></li> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Characterisation" title=""><span>Characterisation</span></a></li> | ||
| + | <li><a href="https://2007.igem.org/Imperial/Cell-Free/Applications" title=""><span>CFS Applications</span></a></li> | ||
| + | |||
| + | </ul> | ||
| + | </div> | ||
| + | <hr /> | ||
| + | <br clear="all"> | ||
| + | </html> | ||
== Cell-Free: A Comparison of ''In Vivo'' and ''In Vitro'' Systems == | == Cell-Free: A Comparison of ''In Vivo'' and ''In Vitro'' Systems == | ||
Revision as of 19:51, 26 October 2007
Cell-Free: A Comparison of In Vivo and In Vitro Systems
| Non-infectious because of non-proliferative nature | Some strains may be pathogenic |
| Process is quick and simple requiring only preparation of cell extract and feeding solution and subsequent addition of DNA template | Process is laborious involving DNA cloning and transformation and protein expression |
| Good control can be achieved easily using modified reaction conditions such as addition of accessory elements or inhibitory factors | Less controllability because of the presence of endogenous substances and because cells do not survive extreme conditions |
| Both plasmid and linear DNAs and can be used as templates for expression | Plasmid DNAs are usually used. Linear DNAs are easily degraded by endogenous nucleases |
| Protein degradation is minimized by adding protease inhibitors | Synthesized proteins may be degraded by endogenous proteases |
| Toxic proteins can be synthesized in large quantities | Synthesis of toxic proteins may kill the cells |
| Proteins containing unnatural amino acids can be achieved | Difficult to produce proteins containing unnatural amino acids |
| Shorter lifespan since system cannot replicate | Longer lifespan since system can replicate |
| More expensive because of the constant need for nutrient and energy supply | Less expensive because of the ability of the system to generate energy from relatively cheap nutrient source |
| Less characterized, less experience of use in the laboratories | Better characterized, more experience of use in the laboratories |
