Imperial/Wet Lab/Protocols
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= Wet Lab: Protocols = | = Wet Lab: Protocols = | ||
| + | Below is a databank of all the protocols that have been used in our projects. | ||
| + | <br><br> | ||
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| + | {| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" | ||
| + | |- style="background:#aabadd; color:Black" align="center" | ||
| + | || <font size=3>'''Fabrication Protocols'''</font>|| <font size=3>'''Testing Protocols'''</font> | ||
| + | |- style="background:#d5dcef; color:Black" valign="top" | ||
| + | | | ||
| + | <font size=3>'''Construct Protocols'''</font> <br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/DNA1.1|Cloning of DNA- MiniPrep]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/DNA1.2|Cloning of DNA- MidiPrep]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/DNA1.3|Cloning of DNA- MaxiPrep]] | ||
| + | |||
| + | <br> | ||
| + | <font size=3>'''Protein Purification'''</font> <br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.5|Purification of LuxR]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.7|Testing of Purified LuxR]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.6|Purification of GFPmut3b]] | ||
| + | |||
| + | <br> | ||
| + | <font size=3>'''Fluorometer Testing'''</font> <br> | ||
| + | |||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.1|Optimsation of Fluorometer]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.3|Calibration Curve for DsRed express]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.4|Degradation Curve]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Prot1.8|DNA concentration experiments]] | ||
| + | |||
| + | <br> | ||
| + | <font size=3>'''Chassis Protocols'''</font> <br> | ||
| + | '''''E. coli'' Extracts'''<br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/CE1.1|Preparation of S30 ''E. coli'' extract]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/CE1.2|Preparation of S12 ''E. coli'' extract]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/Protocols/CE1.3|Preparation of reaction mixture]] | ||
| + | |||
| + | '''Vesicles Formation'''<br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/VF1.1|Vesicles Formation]] | ||
| + | * 2 | ||
| + | |||
| + | | | ||
| + | <font size=3>'''Cell by Date'''</font> <br> | ||
| + | '''Implementation'''<br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/CBD1.1|Initial Testing for DNA constructs of CBD In Vivo]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/CBD1.2|Initial Testing for DNA constructs of CBD In Vitro (Chassis)]] | ||
| + | |||
| + | |||
| + | '''Testing/Validation'''<br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/CBD2.1|Operating Temperature Range (Initial Tests)]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/CBD2.2|Operating Temperature Range (Refined Tests)]] | ||
| + | |||
| + | |||
| + | <br> | ||
| + | <font size=3>'''Infector Detector'''</font> <br> | ||
| + | '''Implementation'''<br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/ID1.1|Initial Testing for DNA constructs of ID In Vivo]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro (Chassis)]] | ||
| + | |||
| + | '''Testing/Validation'''<br> | ||
| + | * [[Imperial/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]] | ||
| + | * [[Imperial/Wet_Lab/Protocols/ID3.1|Final AHL Testing (Standardized)]] | ||
| + | |||
| + | |||
| + | |} | ||
| + | <br clear="all"> | ||
Revision as of 01:47, 22 October 2007
Wet Lab: Protocols
Below is a databank of all the protocols that have been used in our projects.
| Fabrication Protocols | Testing Protocols |
|
Construct Protocols
Vesicles Formation |
Cell by Date
Testing/Validation
|
