Construct Protocols

• Cloning of DNA- MiniPrep
• Cloning of DNA- MidiPrep
• Cloning of DNA- MaxiPrep

Protein Purification

• Purification of LuxR
• Testing of Purified LuxR
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/Construct_2 Results]
• Purification of GFPmut3b
• GFPmut3b Calibration Curve (Wrong, this is the recombinant GFP

Fluorometer Testing

• Optimisation of Fluorometer
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_3.1 Results]
• Measuring and Preventing Evaporation?? Wrong.
• Testing How Fluorescence Changes With Volume
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_1.1 Results]
• Calibration Curve for DsRed express
• Degradation Curve (For GFP? Non-existent)

Chassis Protocols
E. coli Extracts

• Preparation of S30 E. coli extract
• Preparation of S12 E. coli extract
• Preparation of reaction mixture

Vesicles Formation

• Vesicles Formation
• 2

Cell by Date
Implementation

• Initial Testing for DNA constructs of CBD In Vivo
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
• Initial Testing for DNA constructs of CBD In Vitro (Chassis)
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]

Testing/Validation

• Operating Temperature Range (Initial Tests)
• Operating Temperature Range (Refined Tests)
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.1.1 Results + ones for initial operating range]
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results for pT7]
• Detemining System Capabilities - DNA concentration
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
• Packaging protocol?? Results are in OWW.
• Steps protocol?? Results are in OWW.

Infector Detector
Implementation

• Initial Testing for DNA constructs of ID In Vivo
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
• Initial Testing for DNA constructs of ID In Vitro (Chassis)
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]

Testing/Validation

• Preliminary AHL Testing
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.2.1 Results]
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.2.3 Results]
• AHL Sensitivity: Real Life Applications
  • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Biofilm_Detector/TestingValidation Results]
• Final AHL Testing (Standardized)