Imperial/Wet Lab/Protocols

From 2007.igem.org

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|| <font size=3>'''General Protocols'''</font>|| <font size=3>'''Project Specific Protocols'''</font>
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||<font size=2>'''General Experiments'''</font>||<font size=2>'''Testing Experiments'''</font>
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<font size=3>'''Construct Protocols'''</font> <br>
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<font size=2>'''Protein Purification'''</font> <br>
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* [[Imperial/Wet_Lab/Protocols/DNA1.1|Cloning of DNA- MiniPrep]]
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* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and Results
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* [[Imperial/Wet_Lab/Protocols/DNA1.2|Cloning of DNA- MidiPrep]]
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* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and Results
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* [[Imperial/Wet_Lab/Protocols/DNA1.3|Cloning of DNA- MaxiPrep]]
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<br>
<br>
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<font size=3>'''Protein Purification'''</font> <br>
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<font size=2>'''Fluorometer Testing'''</font> <br>
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* [[Imperial/Wet_Lab/Protocols/Prot1.5|Purification of LuxR]]
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* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and Results
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* [[Imperial/Wet_Lab/Protocols/Prot1.7|Testing of Purified LuxR]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/Construct_2 Results]
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* [[Imperial/Wet_Lab/Protocols/Prot1.6|Purification of GFPmut3b]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.9|GFPmut3b Calibration Curve (Wrong, this is the recombinant GFP]]
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<br>
<br>
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<font size=3>'''Fluorometer Testing'''</font> <br>
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<font size=2>'''Calibration Curve'''</font> <br>
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* ''Calibration Curve for GFPmut3b'' [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and Results
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* [[Imperial/Wet_Lab/Protocols/Prot1.1|Optimisation of Fluorometer]]
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* ''Degradation Curve for GFPmut3b'' [[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and Results
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_3.1 Results]
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<br>
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* [[Imperial/Wet_Lab/Protocols/Prot1.5|Measuring and Preventing Evaporation?? Wrong.]]
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<font size=2>'''Chassis Protocols'''</font> <br>
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* [[Imperial/Wet_Lab/Protocols/Prot1.2|Testing How Fluorescence Changes With Volume]]
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* ''Preparation S30 Cell Extract ''[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_1.1 Results]
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* ''Preparation S12 Cell Extract ''[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.3|Calibration Curve for DsRed express]]
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* ''Preparation of Reaction Mixture'' [[Imperial/Wet_Lab/Protocols/Protocols/CE1.3|Protocol]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.4|Degradation Curve (For GFP? Non-existent)]]
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* ''Vesicles Formation'' [[Imperial/Wet_Lab/Protocols/VF1.1|Vesicles Formation]]
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<br>
<br>
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<font size=3>'''Chassis Protocols'''</font> <br>
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'''''E. coli'' Extracts'''<br>
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<font size=2>'''Cell by Date'''</font> <br>
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* [[Imperial/Wet_Lab/Protocols/CE1.1|Preparation of S30 ''E. coli'' extract]]
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* [[Imperial/Wet_Lab/Protocols/CE1.2|Preparation of S12 ''E. coli'' extract]]
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* [[Imperial/Wet_Lab/Protocols/Protocols/CE1.3|Preparation of reaction mixture.. what is this actually??]]
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'''Vesicles Formation'''<br>
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* [[Imperial/Wet_Lab/Protocols/VF1.1|Vesicles Formation]]
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<font size=3>'''Cell by Date'''</font> <br>
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'''Implementation'''<br>
'''Implementation'''<br>
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* [[Imperial/Wet_Lab/Protocols/CBD1.1|Initial Testing for DNA constructs of CBD In Vivo]]
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* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and  [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
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* ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]]
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* [[Imperial/Wet_Lab/Protocols/CBD1.2|Initial Testing for DNA constructs of CBD In Vitro (Chassis)]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
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'''Testing/Validation'''<br>
'''Testing/Validation'''<br>
* [[Imperial/Wet_Lab/Protocols/CBD2.1|Operating Temperature Range (Initial Tests)]]
* [[Imperial/Wet_Lab/Protocols/CBD2.1|Operating Temperature Range (Initial Tests)]]
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* Packaging protocol?? Results are in OWW.
* Packaging protocol?? Results are in OWW.
* Steps protocol?? Results are in OWW.
* Steps protocol?? Results are in OWW.
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<br>
 
<font size=3>'''Infector Detector'''</font> <br>
<font size=3>'''Infector Detector'''</font> <br>
'''Implementation'''<br>
'''Implementation'''<br>
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* [[Imperial/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro (Chassis)]]
* [[Imperial/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro (Chassis)]]
** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]
** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]
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'''Testing/Validation'''<br>
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* [[Imperial/Wet_Lab/Protocols/ID3.1|Final AHL Testing]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.7|Testing of Purified LuxR]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/Construct_2 Results]
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'''Testing/Validation'''<br>
 
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* [[Imperial/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.2.1 Results]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.2.3 Results]
 
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* [[Imperial/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]]
 
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Biofilm_Detector/TestingValidation Results]
 
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* [[Imperial/Wet_Lab/Protocols/ID3.1|Final AHL Testing (Standardized)]]
 
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Revision as of 21:04, 22 October 2007


Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification

  • Purification of LuxR : Protocol and Results
  • Purification of GFPmut3b: Protocol and Results


Fluorometer Testing

  • Optimisation of Fluorometer: Protocol and Results


Calibration Curve

  • Calibration Curve for GFPmut3b Protocol and Results
  • Degradation Curve for GFPmut3b Protocol and Results


Chassis Protocols


Cell by Date
Implementation

  • In Vivo Testing: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
  • Initial In vitro Testing:Protocols
    • [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]

Testing/Validation

Infector Detector
Implementation

Testing/Validation



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