Imperial/Wet Lab/Protocols

From 2007.igem.org

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<font size=2>'''Calibration Curve'''</font> <br>
<font size=2>'''Calibration Curve'''</font> <br>
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* ''Calibration Curve for GFPmut3b'' [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and Results
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* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and Results
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* ''Degradation Curve for GFPmut3b'' [[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and Results
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* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and Results
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<font size=2>'''Chassis Protocols'''</font> <br>
<font size=2>'''Chassis Protocols'''</font> <br>
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* ''Preparation S30 Cell Extract ''[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
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* ''Preparation S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
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* ''Preparation S12 Cell Extract ''[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
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* ''Preparation S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
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* ''Preparation of Reaction Mixture'' [[Imperial/Wet_Lab/Protocols/Protocols/CE1.3|Protocol]]
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* ''Preparation of Reaction Mixture'': [[Imperial/Wet_Lab/Protocols/Protocols/CE1.3|Protocol]]
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* ''Vesicles Formation'' [[Imperial/Wet_Lab/Protocols/VF1.1|Vesicles Formation]]
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* ''Vesicles Formation'' :[[Imperial/Wet_Lab/Protocols/VF1.1|Vesicles Formation]]
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<font size=2>'''pT7 Characterisation'''</font> <br>
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*''pT7 in vitro:'' Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results]
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<font size=2>'''Cell by Date'''</font> <br>
<font size=2>'''Cell by Date'''</font> <br>
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'''Implementation'''<br>
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Implementation<br>
* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and  [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and  [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
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* ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]]
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* ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
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Testing/Validation<br>
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'''Testing/Validation'''<br>
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*''Testing DNA Concentration in vitro'':
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* [[Imperial/Wet_Lab/Protocols/CBD2.1|Operating Temperature Range (Initial Tests)]]
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* ''Operating Temperature Range'' :[[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
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* [[Imperial/Wet_Lab/Protocols/CBD2.2|Operating Temperature Range (Refined Tests)]]
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*''Temperature Steps:'' Protocols and Results
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase2/Results_2.1.1 Results + ones for initial operating range]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results for pT7]
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* [[Imperial/Wet_Lab/Protocols/Prot1.8|Detemining System Capabilities - DNA concentration]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
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* Packaging protocol?? Results are in OWW.
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* Steps protocol?? Results are in OWW.
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<font size=3>'''Infector Detector'''</font> <br>
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'''Implementation'''<br>
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* [[Imperial/Wet_Lab/Protocols/ID1.1|Initial Testing for DNA constructs of ID In Vivo]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
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* [[Imperial/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro (Chassis)]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]
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'''Testing/Validation'''<br>
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* [[Imperial/Wet_Lab/Protocols/ID3.1|Final AHL Testing]]
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* [[Imperial/Wet_Lab/Protocols/Prot1.7|Testing of Purified LuxR]]
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** [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/Construct_2 Results]
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<font size=2>'''Infecter Detector'''</font> <br>
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Implementation<br>
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*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
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* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]
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Testing/Validation<br>
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* ''Testing of Purified LuxR'' :[[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and Results
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* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and Results
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Revision as of 21:16, 22 October 2007


Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification

  • Purification of LuxR : Protocol and Results
  • Purification of GFPmut3b: Protocol and Results


Fluorometer Testing

  • Optimisation of Fluorometer: Protocol and Results


Calibration Curve

  • Calibration Curve for GFPmut3b: Protocol and Results
  • Degradation Curve for GFPmut3b :Protocol and Results


Chassis Protocols


pT7 Characterisation

  • pT7 in vitro: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results]

Cell by Date
Implementation

  • In Vivo Testing: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
  • Initial In vitro Testing:Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]

Testing/Validation

  • Testing DNA Concentration in vitro:
  • Operating Temperature Range :Protocol 1 and Protocol 2 and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
  • Temperature Steps: Protocols and Results

Infecter Detector
Implementation

  • In vivo Testing: Protocol and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
  • Initial In Vitro Testing: Protocol and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]

Testing/Validation

  • Testing of Purified LuxR :Protocol and Results
  • Characterization in vitro - AHL titration : Protocol and Results


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