Imperial/Wet Lab/Protocols

From 2007.igem.org

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<font size=2><font color=green>'''Protein Purification'''</font> <br>
<font size=2><font color=green>'''Protein Purification'''</font> <br>
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* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and Results
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* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[Imperial/Wet_Lab/results/Res1.5|Results]]
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* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and Results
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* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[Imperial/Wet_Lab/results/Res1.6|Results]]
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<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
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* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and Results
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* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[Imperial/Wet_Lab/results/Res1.1|Results]]<br>
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<font size=2><font color=green>'''Calibration Curve'''</font> <br>
<font size=2><font color=green>'''Calibration Curve'''</font> <br>
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* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and Results
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* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[Imperial/Wet_Lab/results/Res1.3|Results]]
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* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and Results
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* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/results/Res1.4|Results]]
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<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
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<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
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*''pT7 in vitro:'' Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results]
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*''pT7 in vitro:'' Protocols and [[Imperial/Wet_Lab/results/Res1.9|Results]]
<font size=2><font color=green>'''Cell by Date'''</font>  
<font size=2><font color=green>'''Cell by Date'''</font>  
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'''Implementation'''
'''Implementation'''
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* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
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* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[Imperial/Wet_Lab/results/CBD1.1|Results]]
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* ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]
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* ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/results/CBD1.2|Results]]
'''Testing/Validation<br>'''
'''Testing/Validation<br>'''
*''Testing DNA Concentration in vitro'':
*''Testing DNA Concentration in vitro'':
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* ''Operating Temperature Range'' :[[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
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* ''Operating Temperature Range'' :[[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and  [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [[Imperial/Wet_Lab/results/CBD2.2|Results]]
*''Temperature Steps:'' Protocols and Results
*''Temperature Steps:'' Protocols and Results
<font size=2><font color=green>'''Infecter Detector'''</font> <br>
<font size=2><font color=green>'''Infecter Detector'''</font> <br>
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Implementation<br>
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'''Implementation<br>'''
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*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
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*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/results/ID1.1|Results]]
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* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]
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* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[Imperial/Wet_Lab/results/ID1.2|Results]]
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Testing/Validation<br>
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'''Testing/Validation<br>'''
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* ''Testing of Purified LuxR'' :[[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and Results
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* ''Testing of Purified LuxR'' :[[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[Imperial/Wet_Lab/results/Res1.7|Results]]
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* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and Results
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* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[Imperial/Wet_Lab/results/ID3.1|Results]]
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Revision as of 21:26, 22 October 2007


Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols


pT7 Characterisation

  • pT7 in vitro: Protocols and Results

Cell by Date
Implementation

Testing/Validation

  • Testing DNA Concentration in vitro:
  • Operating Temperature Range :Protocol 1 and Protocol 2 and Results
  • Temperature Steps: Protocols and Results

Infecter Detector
Implementation

Testing/Validation


Retrieved from "http://2007.igem.org/wiki/index.php/Imperial/Wet_Lab/Protocols"