Imperial/Cell by Date/Implementation
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|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center> | |colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center> | ||
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- | |wifth="20%" style="background-color:#ddeeff"| '''Construct Design''' | + | |wifth="20%" style="background-color:#ddeeff" align="center"| '''Construct Design''' |
- | |width="40%"| [[Image:Imperial CBD Design CBD1.png]] | + | |width="40%"| '''Concept 1'''<br />[[Image:Imperial CBD Design CBD1.png|center]] |
- | |width="40%"| [[Image:Imperial CBD Design CBD2.png]] | + | |width="40%"| '''Concept 2'''<br />[[Image:Imperial CBD Design CBD2.png|center]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' | ||
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|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | ||
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/ | + | * '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results]) |
* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
|width="40%"| | |width="40%"| | ||
- | * '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/ | + | * '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results]) |
* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
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- | | | + | |colspan="3" style="background-color:#eeeeff"| <center>'''Chassis Construct Implementation'''</center> |
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- | |- | + | |width="20%" style="background-color:#ddeeff"| '''Chassis Design''' |
- | | | + | |colspan="2" align="center" |[[Image:IC07_CFS_components.png|300px]] |
- | |width="- | + | |- align="center" |
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability''' | ||
+ | |width="40%"| Possibility of making Homemade S30 Cell Extract. | ||
+ | |width="40%"| Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. | ||
+ | |- align="center" | ||
+ | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Preparation''' | ||
+ | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]) | ||
+ | |width="40%"| '''Preparation''': Done ([[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]) | ||
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+ | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 3: Quality Control''' | ||
+ | |width="40%"| | ||
+ | * '''Testing ''in vitro''''': Done (Protocol and Results) | ||
+ | |width="40%"| | ||
+ | * '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results]) | ||
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+ | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | ||
+ | |width="40%" style="background-color:#ddb99e"| Aborted. <br /> Expression capacity of chassis was low. | ||
+ | |width="40%" style="background-color:#b8efb0"| Ready for Testing | ||
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== Navigation== | == Navigation== | ||
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<center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center> | <center> [https://2007.igem.org/Imperial/Cell_by_Date/Modelling << Modelling ] | Implementation | [https://2007.igem.org/Imperial/Cell_by_Date/Testing Testing/Validation >>]</center> |
Revision as of 13:35, 24 October 2007
Cell by Date: Implementation
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Construct Design | Concept 1 | Concept 2 | |||||
Stage 1: Availability | Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry | Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry. | |||||
Stage 2: Cloning / Amplification | |||||||
Stage 3: Ligation | Not Required | Since [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] was not functional, we made our own promoter and and ligated it to [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
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Stage 4: Purification of Activator Proteins | Not Required | Not Required | |||||
Stage 5: Quality Control |
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Stage 6: Final Implementation Status | Ready for Testing | Ready for Testing |
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Chassis Design | ||
Stage 1: Availability | Possibility of making Homemade S30 Cell Extract. | Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega]. |
Stage 2: Preparation | Preparation: Done (Protocol) | Preparation: Done (Protocol) |
Stage 3: Quality Control |
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Stage 6: Final Implementation Status | Aborted. Expression capacity of chassis was low. | Ready for Testing |