Imperial/Cell-Free/Applications
From 2007.igem.org
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== Our Design == | == Our Design == | ||
| - | + | To get around the problem of having bacteria in contact with food or medical devices in the applications of '''Cell by Date''' and '''Infector Detector''' respectively, we aimed to express reporter DNA constructs within a CFS consisting of cell extract encapsulated in selectively permeable vesicles. Our plan was to first prepare the cell extract and perform '''in-vitro expression''' in bulk solution. In parallel, we would investigate and optimize conditions for '''phospholipid vesicle formation'''. The final stage of our project would involve combining the two, so that protein expression is achieved inside the vesicles. | |
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Revision as of 11:19, 19 October 2007
Our Design
To get around the problem of having bacteria in contact with food or medical devices in the applications of Cell by Date and Infector Detector respectively, we aimed to express reporter DNA constructs within a CFS consisting of cell extract encapsulated in selectively permeable vesicles. Our plan was to first prepare the cell extract and perform in-vitro expression in bulk solution. In parallel, we would investigate and optimize conditions for phospholipid vesicle formation. The final stage of our project would involve combining the two, so that protein expression is achieved inside the vesicles.
Cell-free in Infector Detector
(Specs/Design based)
Cell-free in Cell by Date
(Specs/Design based)
