Imperial

From 2007.igem.org

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<h2>Project Overview</h2>
<h2>Project Overview</h2>
<div class="parabox">Our contribution to the synthetic biology community this year will be the investigation of Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built.  
<div class="parabox">Our contribution to the synthetic biology community this year will be the investigation of Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built.  
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<h2>Our Achievements</h2>
 
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                      <li>Investigated and characterised the in-vitro chassis
 
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                      <li>Designed and modelled a device to detect biofilms and potentially prevent diseases like MRSA
 
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                      <li>Characterised Infector Detector in terms of various AHL concentrations, at room temperature
 
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                      <li>Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system
 
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                      <li>Characterised the pTet-GFP and pT7-GFP construct in-vitro
 
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<h2>Foundational technology - <a href="https://2007.igem.org/Imperial/Cell-Free/Introduction">Cell-Free Systems</a></h2>
<h2>Foundational technology - <a href="https://2007.igem.org/Imperial/Cell-Free/Introduction">Cell-Free Systems</a></h2>
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Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification requirements. Click <a href="https://2007.igem.org/Imperial/Cell-Free/Introduction">here</a> for more details!
 
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      <td valign="top" style="text-align:justify;">Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification requirements. Click <a href="https://2007.igem.org/Imperial/Cell-Free/Introduction">here</a> for more details!<br>
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          <div id="SummaryBox">
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<h2>Main Achievements</h2>
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<div class="parabox">
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                      <ul>
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                      <li>Investigated and characterised the <i>in vitro</i> chassis
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                      <li>Characterised the F2620, pTet-GFP and pT7-GFP construct <i>in vitro</i>
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                      </ul>
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Revision as of 17:16, 19 October 2007

Project Overview

Our contribution to the synthetic biology community this year will be the investigation of Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built.

Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

Main Achievements

  • Designed and modelled a device to detect biofilms and potentially prevent diseases like MRSA
  • Characterised Infector Detector in terms of various AHL concentrations, at room temperature


Image for Specs.
SPECIFICATIONS
Image for Testing/Validation.
TESTING / VALIDATION 		Image for full system.
 Image for Design.
DESIGN

Image for full system.
INFECTOR DETECTOR
Image for Implementation.
IMPLEMENTATION
Image for Modelling.
MODELLING

Foundational technology - Cell-Free Systems

Power your iGEM projects with Cell-Free Systems! Select from the range of options available to suit your specification requirements. Click here for more details!

Main Achievements

  • Investigated and characterised the in vitro chassis
  • Characterised the F2620, pTet-GFP and pT7-GFP construct in vitro

Sub Project - Cell by Date

Cell by Date will report when beef has been out of the fridge for too long. It produces fluorescent proteins at ambient temperatures above 8°C. (more...)

Main Achievements

  • Designed and modelled a device to indicate spoilage of meat/break in the cold chain via the thermal history of the system

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