Imperial/Cell by Date/Implementation

From 2007.igem.org

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__NOTOC__
__NOTOC__
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{|border=1 width=90% align="center"
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|-
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|width="30%"|<center>[[Image:Imperial CBD Design CBD1.png ]]</center>
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|width="--"|Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
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|-
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|<center>[[Image:Imperial CBD Design CBD2.png]]</center>
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|width="--"|Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
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|}
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===Construct Status===
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=====Constitutive tetracyclin promoter=====
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<center>[[Image:Imperial CBD Design CBD1.png]]</center>
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'''Quality Control:'''
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{|border=1 width=90% align="center"
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|-
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|width="20%"|&nbsp;Testing ''in vivo''
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|width="10%" align="center"|Done
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|width="--"|Results can be found at -----
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|-
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|&nbsp;Testing ''in vitro''
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|align="center"|Done
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|Results can be found at the [[Imperial/Cell_by_Date/Testing|testing]] page.
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|-
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|&nbsp;Sequencing
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|align="center"|Pending
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|''Sequence results not obtained yet''
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|}
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=====Constitutive T7 promoter=====
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<center>[[Image:Imperial CBD Design CBD2.png]]</center>
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'''Ligation Strategy:'''
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{|
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|-
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|T7
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|<font size="+3">+</font>
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|GFP
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|<font size="+3">=</font>
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|[[Image:Imperial CBD Design CBD2.png]]
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|}
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'''Quality Control:'''
 +
{|border=1 width=90% align="center"
 +
|-
 +
|width="20%"|&nbsp;Testing ''in vivo''
 +
|width="10%" align="center"|Done
 +
|width="--"|Results can be found at -----
 +
|-
 +
|&nbsp;Testing ''in vitro''
 +
|align="center"|Done
 +
|Results can be found at -----
 +
|-
 +
 +
|&nbsp;Sequencing
 +
|align="center"|Pending
 +
|''Sequence results not obtained yet''
 +
|}
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== Constructs ==
 
-
(Ligation Strategy??)
 
-
(Quality Control??)
 
-
Sequencing of Parts?
 
== Chassis ==
== Chassis ==
cell extract preparation protocols.
cell extract preparation protocols.

Revision as of 17:06, 21 October 2007

Imperial CBD Design CBD1.png
Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
Imperial CBD Design CBD2.png
Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]

Construct Status

Constitutive tetracyclin promoter
Imperial CBD Design CBD1.png

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at the testing page.
 Sequencing Pending Sequence results not obtained yet


Constitutive T7 promoter
Imperial CBD Design CBD2.png

Ligation Strategy:

T7 + GFP = Imperial CBD Design CBD2.png

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at -----
 Sequencing Pending Sequence results not obtained yet



Chassis

cell extract preparation protocols.

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