Imperial/Cell by Date/Implementation
From 2007.igem.org
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(→Constitutive tetracyclin promoter) |
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| - | ===Constitutive | + | ===Constitutive tetR Promoter=== |
<center>[[Image:Imperial CBD Design CBD1.png]]</center> | <center>[[Image:Imperial CBD Design CBD1.png]]</center> | ||
'''Ligation Strategy:''' | '''Ligation Strategy:''' | ||
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|''Sequence results not obtained yet'' | |''Sequence results not obtained yet'' | ||
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===Constitutive T7 promoter=== | ===Constitutive T7 promoter=== | ||
Revision as of 20:50, 21 October 2007
DNA Constructs
 | Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry |
 | Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functinal, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504] |
Constitutive tetR Promoter
Ligation Strategy:
N/A
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at the testing page. |
| Sequencing | Pending | Sequence results not obtained yet |
Constitutive T7 promoter
Ligation Strategy:
| + | 
| = |
|
Quality Control:
| Testing in vivo | Done | Results can be found at ----- |
| Testing in vitro | Done | Results can be found at ----- |
| Sequencing | Pending | Sequence results not obtained yet |
Chassis
cell extract preparation protocols.
