Imperial/Cell by Date/Implementation

From 2007.igem.org

(Difference between revisions)
(DNA Constructs)
Line 2: Line 2:
__NOTOC__
__NOTOC__
-
== DNA Constructs ==
+
= Cell by Date: Implementation =
 +
 
 +
== DNA Construct Status ==
{|border=1 width=90% align="center"
{|border=1 width=90% align="center"
Line 10: Line 12:
|-
|-
|<center>[[Image:Imperial CBD Design CBD2.png]]</center>
|<center>[[Image:Imperial CBD Design CBD2.png]]</center>
-
|width="--"|Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
+
|width="--"|Notes: The part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]
|}
|}
<br clear="all">
<br clear="all">
Line 68: Line 70:
<br clear="all">
<br clear="all">
-
== Chassis ==
+
== Chassis Status ==
cell extract preparation protocols.
cell extract preparation protocols.

Revision as of 20:56, 21 October 2007


Cell by Date: Implementation

DNA Construct Status

Imperial CBD Design CBD1.png
Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
Imperial CBD Design CBD2.png
Notes: The part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Constitutive tetR Promoter

Imperial CBD Design CBD1.png

Ligation Strategy:

N/A

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at the testing page.
 Sequencing Pending Sequence results not obtained yet

Constitutive T7 Promoter

Imperial CBD Design CBD2.png

Ligation Strategy:

Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.png

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at -----
 Sequencing Pending Sequence results not obtained yet


Chassis Status

cell extract preparation protocols.