Imperial/Wet Lab/Protocols
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<font size=2><font color=green>'''Protein Purification'''</font> <br> | <font size=2><font color=green>'''Protein Purification'''</font> <br> | ||
| - | * ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[Imperial/Wet_Lab/results/Res1.5|Results]] | + | * ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.5|Results]] |
| - | * ''Purification of GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[Imperial/Wet_Lab/results/Res1.6|Results]] | + | * ''Purification of GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.6|Results]] |
<br> | <br> | ||
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br> | <font size=2><font color=green>'''Fluorometer Testing'''</font> <br> | ||
| - | * ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[Imperial/Wet_Lab/results/Res1.1|Results]]<br> | + | * ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.1|Results]]<br> |
<font size=2><font color=green>'''Calibration Curve'''</font> <br> | <font size=2><font color=green>'''Calibration Curve'''</font> <br> | ||
| - | * ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[Imperial/Wet_Lab/results/Res1.3|Results]] | + | * ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.3|Results]] |
| - | * ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/results/Res1.4|Results]] | + | * ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.4|Results]] |
<br> | <br> | ||
<font size=2><font color=green>'''Chassis Protocols'''</font> <br> | <font size=2><font color=green>'''Chassis Protocols'''</font> <br> | ||
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<font size=2><font color=green>'''pT7 Characterisation'''</font> <br> | <font size=2><font color=green>'''pT7 Characterisation'''</font> <br> | ||
| - | *''pT7 in vitro:'' Protocols and [[Imperial/Wet_Lab/results/Res1.9|Results]] | + | *''pT7 in vitro:'' Protocols and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.9|Results]] |
<font size=2>'''Cell by Date'''</font> | <font size=2>'''Cell by Date'''</font> | ||
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<font color=green>'''Implementation'''</font> | <font color=green>'''Implementation'''</font> | ||
<br> | <br> | ||
| - | * ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[Imperial/Wet_Lab/results/CBD1.1|Results]] | + | * ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.1|Results]] |
| - | * ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/results/CBD1.2|Results]] | + | * ''Initial In vitro Testing'':[[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD1.2|Results]] |
<font color=green>'''Testing/Validation<br>'''</font> | <font color=green>'''Testing/Validation<br>'''</font> | ||
| - | *''Testing DNA Concentration in vitro'': | + | *''Testing DNA Concentration in vitro'': Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/CBD4 |Results]] |
| - | * ''Operating Temperature Range'' :[[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [[Imperial/Wet_Lab/results/CBD2.2|Results]] | + | * ''Operating Temperature Range'' :[[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [[IGEM:Imperial/2007/Wet_Lab/results/CBD2.2|Results]] |
*''Temperature Steps:'' Protocols and Results | *''Temperature Steps:'' Protocols and Results | ||
<font size=2>'''Infecter Detector'''</font> <br> | <font size=2>'''Infecter Detector'''</font> <br> | ||
<font color=green>'''Implementation<br>'''</font> | <font color=green>'''Implementation<br>'''</font> | ||
| - | *''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/results/ID1.1|Results]] | + | *''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.1|Results]] |
| - | * ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[Imperial/Wet_Lab/results/ID1.2|Results]] | + | * ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID1.2|Results]] |
<font color=green>'''Testing/Validation<br>'''</font> | <font color=green>'''Testing/Validation<br>'''</font> | ||
| - | * ''Testing of Purified LuxR'' :[[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[Imperial/Wet_Lab/results/Res1.7|Results]] | + | *''Testing of DNA concentration'' : Protocol and [[IGEM:Imperial/2007/Wet_Lab/results/Res2.1|Results]] |
| - | * ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[Imperial/Wet_Lab/results/ID3.1|Results]] | + | * ''Testing of Purified LuxR'' :[[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.7|Results]] |
| + | * ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/ID3.1|Results]] | ||
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Revision as of 21:55, 22 October 2007
Wet Lab: Protocols
Below is a databank of all the protocols that have been used in our projects.
| General Experiments | Testing Experiments |
|
Protein Purification
Calibration Curve
|
pT7 Characterisation
Cell by Date
Testing/Validation
Infecter Detector Testing/Validation |
