Imperial/Wet Lab/Protocols

From 2007.igem.org

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<font size=2><font color=green>'''Protein Purification'''</font> <br>
<font size=2><font color=green>'''Protein Purification'''</font> <br>
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* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [IGEM:Imperial/2007/Wet_Lab/results/Res1.5|Results]
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* ''Purification of LuxR'' : [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]
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* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.6|Results]]
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* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.6 Results]
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<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
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* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.1|Results]]<br>
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* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.1 Results]<br>
<font size=2><font color=green>'''Calibration Curve'''</font> <br>
<font size=2><font color=green>'''Calibration Curve'''</font> <br>
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* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.3|Results]]
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* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.3 Results]
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* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.4|Results]]
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* ''Degradation Curve for GFPmut3b'' :[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.4 Results]
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<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
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<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
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*''pT7 in vitro:'' Protocols and [[IGEM:Imperial/2007/Wet_Lab/results/Res1.9|Results]]
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*''pT7 in vitro:'' Protocols and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.9 Results]
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<font size=2>'''Cell by Date'''</font>  
<font size=2>'''Cell by Date'''</font>  
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Revision as of 22:03, 22 October 2007


Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification

  • Purification of LuxR : Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]
  • Purification of GFPmut3b: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.6 Results]


Fluorometer Testing

  • Optimisation of Fluorometer: Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.1 Results]

Calibration Curve

  • Calibration Curve for GFPmut3b: Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.3 Results]
  • Degradation Curve for GFPmut3b :Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.4 Results]


Chassis Protocols


pT7 Characterisation

  • pT7 in vitro: Protocols and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.9 Results]

Cell by Date
Implementation

Testing/Validation

Infecter Detector
Implementation

Testing/Validation


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