From 2007.igem.org

Revision as of 13:11, 25 October 2007 (view source) Maira (Talk | contribs) m (→'''10°C''') ← Older edit		Revision as of 23:17, 25 October 2007 (view source) Maira (Talk | contribs) m Newer edit →
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	__NOTOC__		__NOTOC__
-	=Test for operation at different temperatures for pTet-GFPmut3b=	+
		+	=Wet Lab: ResultsTest for optimum packaging and sampling techniques=
	== [http://partsregistry.org/Part:BBa_I13522 '''pTet-GFP''']==		== [http://partsregistry.org/Part:BBa_I13522 '''pTet-GFP''']==
	==Aim==		==Aim==
-	The aim of these experiments was to firgure out the operating range of the pTet-GFP construct in-vitro. The temperatures the construct was tested in are: 8°C, 10°C, 20°C and 37°C.	+	*Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
-		+	*Determine a suitable sampling technique which disrupts the reaction to a very minimum
-	<br>'''31-08-2007'''	+	<br>'''??-??-2007'''
-		+
-	==='''10&deg;C'''===	+
-	pTet was tested at 10<sup>o</sup>C for 4 hours. The results show no expression of pTet-GFP. After this experiment we took the samples out and put them at room temperature over night, this is to see if the low temperature permanently inactivate the in vitro chassis or DNA.	+
-		+
-	<br clear="all">	+
-	{| border="2" style="background:#ABCDEF;"	+
-	| [[Media:IC 2007 PTet-in-vitro-10oC.xls|Complete set of results and raw data ]]	+
-	|}	+
-		+
-	==='''20&deg;C'''===	+
-	pTet was tested at 20&deg;C for two days.	+
-	<br clear="all">	+
-	{| border="2" style="background:#ABCDEF;"	+
-	| [[Media:IC 2007 Ptet 20oC-1.xls|Complete set of results and raw data ]]	+
-	|}	+
-		+
-	==='''37&deg;C'''===	+
-	pTet was tested at 37&deg;C for two days and a staggered experiment was carried out to minimise the missed measurements over night.	+
-	<br clear="all">	+
-	{| border="2" style="background:#ABCDEF;"	+
-	| [[Media:IC 2007 Graph 37oC.xls|Complete set of results and raw data ]]	+
-	|}	+

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Revision as of 23:17, 25 October 2007

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Wet Lab: ResultsTest for optimum packaging and sampling techniques

[http://partsregistry.org/Part:BBa_I13522 pTet-GFP]

Aim

• Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
• Determine a suitable sampling technique which disrupts the reaction to a very minimum

??-??-2007