Imperial/Wet Lab/Results/CBD2.1

From 2007.igem.org

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==Aims==
 
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To determine if the of [http://partsregistry.org/Part:BBa_T9002 '''pTet-LuxR-pLux-GFPmut3b'''] construct works ''in vivo''.
 

Revision as of 23:34, 25 October 2007


Wet Lab: ResultsTest for optimum packaging and sampling techniques

[http://partsregistry.org/Part:BBa_I13522 pTet-GFP]

Aim

  • Determine an optimum packaging technique for the experiments, that minimises the evaporation of the reaction mixture.
  • Determine a suitable sampling technique which disrupts the reaction to a very minimum


??-??-2007


Materials and Methods

See Protocols page
Tested on (Insert link)

Results


[[Image:|thumb|600px|Fig.1.1:pTet-LuxR-pLux-GFPmut3b in vivo - ]]

Controls:

  • Negative Control - 96 well plate with singl layered tape and no pipetting (same experimental conditions as have them usually)

Constants:

  • Temperature - 37°C
  • Volume of Cells sampled - 60µl


Raw Data

Discussion

Conclusion