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Revision as of 01:43, 27 October 2007

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Calibration Curve: GFPmut3b in vitro vs Fluorescence

Aims

To construct a calibration curve of GFPmut3b in the in vitro chassis to enable the conversion of Fluorescence to more useful units.

Materials and Methods

Link to the Protocol

Results

Figure 1.1 - Concentration vs Fluorescence	Figure 1.2 - Moles vs Fluorescence
Figure 1.3 - Molecules vs Fluorescence	Figure 1.4 - Molecules per plasmid vs Fluorescence

Raw Data

• Raw Data excel file

Discussion

The results obtained fit a linear plot very well.

Conclusion

Now that we have a calibration curve in terms of units of fluorescence, it is possible for us to convert the results obtained in terms of fluorescence to molecules produced. This would make the results more generic and thus the transfer function of the constructs can be obtained which is independant of the reporter attached at the end of the construct.

The conversion from one unit to another can be done using this conversion method.