From 2007.igem.org
yesterday -- tomorrow
Expression test of DapA promotor by differential DAP repression
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products
|
| Number
| Name
| Description
| Products in use
| Date
|
| MP15
| Att B
| Lambda Att B rev within pSB2K3
|
| August 30
|
| MP16
| Att P
| Lambda Att P within pSB2K3
|
| August 30
|
Strains (glycerol stock)
| Strains glycerol stock
|
| Number
| Name
| Description
| Verified clone
| Date
|
| S46
| MP15
| Lambda Att B rev within pSB2K3
|
| August 30
|
| S47
| MP16
| Lambda Att P within pSB2K3
|
| August 30
|
Digestions
we digest:
- MP1.1 as Bw vector with Spe1 & Pst1
- MP15 as Bw insert with Xba1 & Pst1
- MP16 as Bw insert with Xba1 & Pst1
Protocol:
ADN: 20µL
BSA: 0.5µL
NE Buffer 2: 5µL
Enzyme 1: 2µL
Enzyme 2: 2µL
H2O: 20.5µL
