From 2007.igem.org

• Home
  • Site Map
  • Meet the Team
  • Fun with iGEM
  • IcGEMs@OpenWetware
  • Acknowledgements
• Infector Detector
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • F2620 Comparison
  • Conclusion
• Cell by Date
  • Introduction
  • Specification
  • Design
  • Modelling
  • Implementation
  • Testing/Validation
  • Conclusion
• Cell-Free
  • What is Cell-Free?
  • Cell-Free Vs. Cell
  • Our Contributions
  • Characterisation
  • Applications
• Wet Lab
  • Lab Notebook
  • Protocols & Results
  • DNA Constructs
• Dry Lab
  • Modelling
  • Data Analysis
  • Software

Infector Detector: Design

Design Overview

Property	Value	Design Solution	System Level
Health Regulations	System Must not be living replicating bacteria	Use a Cell Free System e.g. Promega's S30 Cell Extract	Chassis
Lifespan	System must have a shelf life of 7 days	Protease Inhibitor of Cell Extract Should ensure degradation of Visual Reporter is Minimal Proper Packaging should ensure that evaporation of Cell Free system is so low that system can surive for 7 days	Chassis
Inputs	System must be sensitive to AHL concentration between 5-50nM	Sensitive AHL receiver to detect low AHL levels	Construct

Gene Construct

Chassis Selection