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• Cell by Date
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DNA Constructs

	Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
	Notes: the part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]

Constitutive tetR Promoter

Ligation Strategy:

N/A

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at the testing page.
Sequencing	Pending	Sequence results not obtained yet

Constitutive T7 Promoter

Ligation Strategy:

+

=

Quality Control:

Testing in vivo	Done	Results can be found at -----
Testing in vitro	Done	Results can be found at -----
Sequencing	Pending	Sequence results not obtained yet

Chassis

cell extract preparation protocols.