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Infector Detector: Specifications

The system must be able to detect the presence of biofilms on urinary catheters by detection of AHL, at a minimum concentration of 5nM, and report with a visual signal within 3 hours. It must work within a temperature range of 20°-30°C, be portable and easy to use, have a shelf life of at least seven days, and must not be harmful or infectious.

Property	Value
Inputs	System must be sensitive to AHL concentration between 5-50nM
Outputs	System must give a visual signal if bacteria is present
Response Time	System needs to have a response time under 3 hour
Operating Conditions	System must operate within temperature 20-30°C
Health & Safety	System Must not be living replicating bacteria, and in any way harmful or infectious.
Lifespan	System must have a shelf life of 7 days
Packaging	System must be portable and convenient to use

Specifications in detail

• Input: AHL 5-50nM

It is known that, in Pseudomonas aeruginosa biofilms, the concentration of AHL is at least 5nM. (1) Therefore, if the system can successfully report the presence of this concentration, it should be able to detect such biofilms.

• Output: Visible fluorescent protein

In order for the system to be used by a nurse, without needing any special equipment, the output signal must be visible.

• Response Time: < 3 hours

Given that biofilms grow and spread in a period of hours and days, the response time of the system must be as short as possible. The threshold of three hours is achievable in the time constraints of protein expression systems, yet it is short enough in comparison to the growth properties of biofilms.

• Operating conditions: Temperatures from 20° to 30°C
• Health & safety: Non-living, non-infectious
• Packaging and shelf-life of 7 days

References

1. Charlton, TS, et al. A novel and sensitive method for the quantification of N-3-oxoacyl homoserine lactones using gas chromatography–mass spectrometry: application to a model bacterial biofilm. Environmental Microbiology 2 (5), 530–541. October 2000.